Synergistic effects of zeaxanthin and its binding protein in the prevention of lipid membrane oxidation

被引:61
作者
Bhosale, P [1 ]
Bernstein, PS [1 ]
机构
[1] Univ Utah, Sch Med, Dept Ophthalmol & Visual Sci, Moran Eye Ctr, Salt Lake City, UT 84132 USA
来源
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | 2005年 / 1740卷 / 02期
关键词
zeaxanthin; binding protein; antioxidant; lipid; carotenoid;
D O I
10.1016/j.bbadis.2005.02.002
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
There is growing evidence that high levels of the macular xanthophyll carotenoids lutein and zeaxanthin may be protective against visual loss due to age-related macular degeneration, but the actual mechanisms of their protective effects are still poorly understood. We have recently purified, identified and characterized a pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eye which specifically and saturably binds to the two forms of zeaxanthin endogenously found in the foveal region. In this report, we studied the synergistic antioxidant role of zeaxanthin and GSTP1 in egg yolk phosphatidylcholine (EYPC) liposomes using hydrophilic 2,2-azobis(2-methyl-propionamidine) dihydrochloride (AAPH) and lipophilic 2,2'-azobis(2,4-dimethylvaleronitrile) (AMVN) as lipid peroxyl radical generators. The two zeaxantbin diastereomers displayed synergistic antioxidant effects against both azo lipid peroxyl radical generators when bound to GSTP1. In the presence of GSTP1, nondietary (3R,3'S-meso)-zeaxantbin was observed to be a better antioxidant than dietary (3R,3'R)-zeaxanthin. This effect was found to be independent of the presence of glutathione. Carotenoid degradation profiles indicated that the zeaxanthin diastereomers in association with GSTP1 were more resistant to degradation which may account for the synergistic antioxidant effects. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:116 / 121
页数:6
相关论文
共 30 条
[1]   The role of oxidative stress in the pathogenesis of age-related macular degeneration [J].
Beatty, S ;
Koh, HH ;
Henson, D ;
Boulton, M .
SURVEY OF OPHTHALMOLOGY, 2000, 45 (02) :115-134
[2]  
Beatty S, 2001, INVEST OPHTH VIS SCI, V42, P439
[3]  
Bernstein PS, 1997, INVEST OPHTH VIS SCI, V38, P167
[4]   Resonance Raman measurement of macular carotenoids in normal subjects and in age-related macular degeneration patients [J].
Bernstein, PS ;
Zhao, DY ;
Wintch, SW ;
Ermakov, IV ;
McClane, RW ;
Gellermann, W .
OPHTHALMOLOGY, 2002, 109 (10) :1780-1787
[5]   Identification and quantitation of carotenoids and their metabolites in the tissues of the human eye [J].
Bernstein, PS ;
Khachik, F ;
Carvalho, LS ;
Muir, GJ ;
Zhao, DY ;
Katz, NB .
EXPERIMENTAL EYE RESEARCH, 2001, 72 (03) :215-223
[6]   Identification and characterization of a Pi isoform of glutathione S-transferase (GSTP1) as a zeaxanthin-binding protein in the macula of the human eye [J].
Bhosale, P ;
Larson, AJ ;
Frederick, JM ;
Southwick, K ;
Thulin, CD ;
Bernstein, PS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (47) :49447-49454
[7]  
Billsten HH, 2003, PHOTOCHEM PHOTOBIOL, V78, P138, DOI 10.1562/0031-8655(2003)078<0138:PPOXIC>2.0.CO
[8]  
2
[9]   NUTRIENT SOURCES OF PROVITAMIN-A CAROTENOIDS IN THE AMERICAN DIET [J].
BLOCK, G .
AMERICAN JOURNAL OF EPIDEMIOLOGY, 1994, 139 (03) :290-293
[10]   Distribution of lutein and zeaxanthin stereoisomers in the human retina [J].
Bone, RA ;
Landrum, JT ;
Friedes, LM ;
Gomez, CM ;
Kilburn, MD ;
Menendez, E ;
Vidal, I ;
Wang, WL .
EXPERIMENTAL EYE RESEARCH, 1997, 64 (02) :211-218