Calibrated mitotic oscillator drives motile ciliogenesis

被引:58
作者
Al Jord, Adel [1 ,2 ,3 ]
Shihavuddin, Asm [1 ,2 ,3 ]
d'Aout, Raphael Servignat [1 ,2 ,3 ]
Faucourt, Marion [1 ,2 ,3 ]
Genovesio, Auguste [1 ,2 ,3 ]
Karaiskou, Anthi [4 ]
Sobczak-Thepot, Joelle [4 ]
Spassky, Nathalie [1 ,2 ,3 ]
Meunier, Alice [1 ,2 ,3 ]
机构
[1] PSL Res Univ, Ecole Normale Super IBENS, Inst Biol, F-75005 Paris, France
[2] CNRS, UMR 8197, F-75005 Paris, France
[3] INSERM, U1024, F-75005 Paris, France
[4] UPMC Paris 06, Sorbonne Univ, INSERM, CRSA, F-75012 Paris, France
基金
欧洲研究理事会;
关键词
CENTROSOME AMPLIFICATION; HUMAN-CELLS; ACTIVATION; PHOSPHORYLATION; APC/C; CYCLE; DUPLICATION; MECHANISM; NUCLEAR; MITOSIS;
D O I
10.1126/science.aan8311
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Cell division and differentiation depend on massive and rapid organelle remodeling. The mitotic oscillator, centered on the cyclin-dependent kinase 1-anaphase-promoting complex/cyclosome (CDK1-APC/C) axis, spatiotemporally coordinates this reorganization in dividing cells. Here we discovered that nondividing cells could also implement this mitotic clocklike regulatory circuit to orchestrate subcellular reorganization associated with differentiation. We probed centriole amplification in differentiating mouse-brain multiciliated cells. These postmitotic progenitors fine-tuned mitotic oscillator activity to drive the orderly progression of centriole production, maturation, and motile ciliation while avoiding the mitosis commitment threshold. Insufficient CDK1 activity hindered differentiation, whereas excessive activity accelerated differentiation yet drove postmitotic progenitors into mitosis. Thus, postmitotic cells can redeploy and calibrate the mitotic oscillator to uncouple cytoplasmic from nuclear dynamics for organelle remodeling associated with differentiation.
引用
收藏
页码:803 / 806
页数:4
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