Inhibition of lipopolysaccharide-induced inflammatory responses by piperine

被引:84
作者
Bae, Gi-Sang [1 ]
Kim, Min-Sun [1 ]
Jung, Won-Seok [2 ]
Seo, Sang-Wan [3 ]
Yun, Seung-Won [3 ]
Kim, Sung Gyu [3 ]
Park, Rae-Kil [4 ,5 ]
Kim, Eun-Cheol [6 ]
Song, Ho-Joon [1 ]
Park, Sung-Joo [1 ]
机构
[1] Wonkwang Univ, Dept Herbol, Sch Oriental Med, Iksan 540749, Jeonbuk, South Korea
[2] Jeonnam Dev Inst Korean Tradit Med, Jangheung 529851, Jeonnam, South Korea
[3] ChungBuk Oriental Med Ctr, Jecheon 390250, Chungbuk, South Korea
[4] Wonkwang Univ, Vestibulocochlear Res Ctr, Coll Med, Jeonbuk, South Korea
[5] Wonkwang Univ, Dept Microbiol, Coll Med, Jeonbuk, South Korea
[6] Wonkwang Univ, Dept Oral & Maxillofacial Pathol, Dent Coll ECK, Iksan 540749, Jeonbuk, South Korea
关键词
Type; 1; interferon; Endotoxin shock; Interferon regulatory factor; Piperine; Signal transducer and activator of transcription; NF-KAPPA-B; GRAM-NEGATIVE BACTERIA; GENE-EXPRESSION; TNF-ALPHA; INTERFERON-GAMMA; ENDOTOXIC-SHOCK; I INTERFERONS; MICE; RECEPTOR; ACTIVATION;
D O I
10.1016/j.ejphar.2010.05.026
中图分类号
R9 [药学];
学科分类号
100702 [药剂学];
摘要
Piperine, a main component of Piper longum Linn. and Piper nigrum Linn., is a plant alkaloid with a long history of medical use. Piperine exhibits anti-inflammatory activity; however, the underlying mechanism remains unknown. We examined the effects of piperine on lipopolysaccharide (LPS)-induced inflammatory responses. Administration of piperine inhibited LPS-induced endotoxin shock, leukocyte accumulation and the production of tumor necrosis factor-alpha (TNF-alpha), but not of interleukin (IL)-1 beta and IL-6. In peritoneal macrophages, piperine inhibited LPS/poly (I:C)/CpG-ODN-induced TNF-alpha production. Piperine also inhibited LPS-induced endotoxin shock in TNF-alpha knockout (KO) mice. To clarify the inhibitory mechanism of LPS-induced endotoxin shock, type 1 interferon (IFN) mRNA expression was determined. Piperine inhibited LPS-induced expression of type 1 IFN mRNA. Piperine inhibited the levels of interferon regulatory factor (IRF)-1 and IRF-7 mRNA, and the phosphorylation and nuclear translocation of IRF-3. Piperine also reduced activation of signal transducer and activator of transcription (STAT)-1. In addition, activation of STAT-1 was inhibited in IFN-alpha/beta-treated cells by piperine. These results suggest that piperine inhibits LPS-induced endotoxin shock through inhibition of type 1 IFN production. (C) 2010 Elsevier B.V. All rights reserved.
引用
收藏
页码:154 / 162
页数:9
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