High-throughput genotyping for a polymorphism linked to soybean cyst nematode resistance gene Rhg4 by using Taqman™ probes

被引:25
作者
Meksem, K [1 ]
Ruben, E [1 ]
Hyten, DL [1 ]
Schmidt, ME [1 ]
Lightfoot, DA [1 ]
机构
[1] So Illinois Univ, Ctr Excellence Soybean Res Teaching & Outreach, Dept Plant Soil & Gen Agr, Carbondale, IL 62901 USA
关键词
Taqman (TM); genetic mapping; marker-assisted breeding; SCN; Rhg4;
D O I
10.1023/A:1009610009663
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
An individual soybean breeder call generate over one hundred thousand new genotypes each year. The efficiency of selection in these populations could be improved if these genotypes were effectively screened with one DNA marker that identified an important gene, and if laboratory throughput was high and costs were low. Our aim was to develop a rapid genotyping procedure for resistance to the soybean cyst nematode. A high-throughput genotyping method was developed with fluorogenic probes to distinguish between two insertion polymorphisms in alleles of an AFLP market that is located about 50 kbp From the Rhg4 gene candidate. The assay uses the 5' exonuclease activity of Taq polymerase in conjunction with fluorogenic probes fur each allele. The method can be used for scoring the polymorphism in a recombinant inbred line population and for screening parent lines in a breeding program. The Taqman(TM) method of determining genotype was accurate in 90% of scores in the RIL population compared to 95% accuracy with electrophoresis. Among 94 cultivars that are patents in our breeding program allele 2 that is derived from the sources of resistance to SCN was common in resistant cultivars (30 of 56) but rare in susceptible cultivars (3 of 38). Therefore, this: method can be applied to automated large-scale genotyping for soybean breeding programs.
引用
收藏
页码:63 / 71
页数:9
相关论文
共 41 条
  • [1] Bell-Johnson B., 1998, Soybean Genetics Newsletter, V25, P115
  • [2] BERNARD RL, 1988, USDA TECH B, V796
  • [3] Brown R. H., 1987, PRINCIPLES PRACTICE, P179
  • [4] Association of loci underlying field resistance to soybean sudden death syndrome (SDS) and cyst nematode (SCN) race 3
    Chang, SJC
    Doubler, TW
    Kilo, VY
    AbuThredeih, J
    Prabhu, R
    Freire, V
    Suttner, R
    Klein, J
    Schmidt, ME
    Gibson, PT
    Lightfoot, DA
    [J]. CROP SCIENCE, 1997, 37 (03) : 965 - 971
  • [5] Trigonelline concentrations in salt stressed leaves of cultivated Glycine max
    Cho, YK
    Lightfoot, DA
    Wood, AJ
    [J]. PHYTOCHEMISTRY, 1999, 52 (07) : 1235 - 1238
  • [6] Targeted comparative genome analysis and qualitative mapping of a major partial-resistance gene to the soybean cyst nematode
    Concibido, VC
    Young, ND
    Lange, DA
    Denny, RL
    Danesh, D
    Orf, JH
    [J]. THEORETICAL AND APPLIED GENETICS, 1996, 93 (1-2) : 234 - 241
  • [7] Genome mapping of soybean cyst nematode resistance genes in 'Peking', PI 90763, and PI 88788 using DNA markers
    Concibido, VC
    Lange, DA
    Denny, RL
    Orf, JH
    Young, ND
    [J]. CROP SCIENCE, 1997, 37 (01) : 258 - 264
  • [8] Two simple sequence repeat markers to select for soybean cyst nematode resistance coditioned by the rhg1 locus
    Cregan, PB
    Mudge, J
    Fickus, EW
    Danesh, D
    Denny, R
    Young, ND
    [J]. THEORETICAL AND APPLIED GENETICS, 1999, 99 (05) : 811 - 818
  • [9] Targeted isolation of simple sequence repeat markers through the use of bacterial artificial chromosomes
    Cregan, PB
    Mudge, J
    Fickus, EW
    Marek, LF
    Danesh, D
    Denny, R
    Shoemaker, RC
    Matthews, BF
    Jarvik, T
    Young, ND
    [J]. THEORETICAL AND APPLIED GENETICS, 1999, 98 (6-7) : 919 - 928
  • [10] An integrated genetic linkage map of the soybean genome
    Cregan, PB
    Jarvik, T
    Bush, AL
    Shoemaker, RC
    Lark, KG
    Kahler, AL
    Kaya, N
    VanToai, TT
    Lohnes, DG
    Chung, L
    Specht, JE
    [J]. CROP SCIENCE, 1999, 39 (05) : 1464 - 1490