One step isolation and purification of liquiritigenin and isoliquiritigenin from Glycyrrhiza uralensis Risch using high-speed counter-current chromatography

被引：78

作者：

Ma, CJ

Li, GS

Zhang, DL

Liu, K

Fan, X ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Shandong Engn Res Ctr Nat Drugs, Yantai 264003, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY A | 2005年 / 1078卷 / 1-2期

关键词：

counter-current chromatography; Glycyrrhiza uralensis; plant materials; pharmaceutical analysis; liquiritigenin; isoliquiritigenin;

D O I：

10.1016/j.chroma.2005.01.053

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

High-speed counter-current chromatography (HSCCC) technique in semi-preparative scale has been successfully applied to the separation of bioactive flavonoid compounds, liquiritigenin and isoliquiritigenin in one step from the crude extract of Glycyrrhiza uralensis Risch. The HSCCC was performed using a two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water (2:2:1:0.6:2, v/v). Yields of liquiritigenin (98.9% purity) and isoliquiritigenin (98.3% purity) obtained were 0.52% and 0.32%. Chemical structures of the purified liquiritigenin and isoliquiritigenin were identified by electrospray ionization-MS (ESI-MS) and NMR analysis. (c) 2005 Published by Elsevier B.V.

引用

页码：188 / 192

页数：5

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