Comparative cytogenetic and DNA flow cytometric analysis of 242 primary breast carcinomas

被引:11
作者
Adeyinka, A
Baldetorp, B
Mertens, F [1 ]
Olsson, H
Johannsson, O
Heim, S
Pandis, N
机构
[1] Univ Lund Hosp, Dept Clin Genet, SE-22185 Lund, Sweden
[2] Univ Lund Hosp, Dept Oncol, SE-22185 Lund, Sweden
[3] St Savas Hosp, Dept Genet, Athens 11522, Greece
[4] Norwegian Radium Hosp, Dept Canc Genet, N-0310 Oslo, Norway
关键词
D O I
10.1016/S0165-4608(03)00190-0
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The cytogenetic and DNA flow cytometric findings in 242 breast carcinomas were compared. The combined use of both techniques improved the detection of abnormal cell populations from 65% by cytogenetic analysis alone and 59% by DNA flow cytometric analysis alone to 84%. Informative and comparable cytogenetic and flow cytometric data were obtained for 155 tumors. Among these 155 tumors, there was good concordance (64%) between the estimates of genomic changes by the two methods. Most discrepancies were among the DNA-diploid cases, where cytogenetic analysis detected small genomic changes. There were, however, also some exceptions in which large genomic changes detected by one method were missed by the other. Of the specific breast cancer-associated cytogenetic aberrations subjected to separate correlation analysis, polysomy for chromosome 20 was significantly associated with a high S-phase fraction, whereas loss of the long arm of chromosome 16 and/or the presence of a der(I; 16) were significantly associated with a low S-phase fraction. Our data show that cytogenetic and DNA flow cytometric analyses of breast carcinomas give largely comparable results, and that combining data from both methods significantly improves the information obtained by either technique used alone on the genetic abnormalities in these tumors. (C) 2003 Elsevier Inc. All rights reserved.
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收藏
页码:62 / 67
页数:6
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