Identification of hypoxia-responsive genes in a dopaminergic cell line by subtractive cDNA libraries and microarray analysis

被引：13

作者：

Beitner-Johnson, D ^{[1
]}

Seta, K ^{[1
]}

Yuan, Y ^{[1
]}

Kim, HW ^{[1
]}

Rust, RT ^{[1
]}

Conrad, PW ^{[1
]}

Kobayashi, S ^{[1
]}

Millhorn, DE ^{[1
]}

机构：

[1] Univ Cincinnati, Dept Cellular & Mol Physiol, Cincinnati, OH 45267 USA

来源：

PARKINSONISM & RELATED DISORDERS | 2001年 / 7卷 / 03期

基金：

美国国家卫生研究院;

关键词：

oxygen/pheochromocytoma; genomics; tyrosine hydroxylase; carotid body; type I cells;

D O I：

10.1016/S1353-8020(00)00070-5

中图分类号：

R74 [神经病学与精神病学];

学科分类号：

摘要：

Transplantation of dopamine-secreting cells harvested from fetal mesencephalon directly into the striatum has had limited success as a therapy for Parkinson's disease. A major problem is that the majority of the cells die during the first 3 weeks following transplantation. Hypoxia in the tissue surrounding the graft is a potential cause of the cell death. We have used subtractive cDNA libraries and microarray analysis to identify the gene expression profile that regulates tolerance to hypoxia. An improved understanding of the molecular basis of hypoxia-tolerance may allow investigators to engineer cells that can survive in the hypoxic environment of the brain parenchyma following transplantation. (C) 2001 Published by Elsevier Science Ltd.

引用

页码：273 / 281

页数：9

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