TNPO3 Is Required for HIV-1 Replication after Nuclear Import but prior to Integration and Binds the HIV-1 Core

被引：73

作者：

Valle-Casuso, Jose Carlos ^{[1
]}

Di Nunzio, Francesca ^{[1
]}

Yang, Yang ^{[1
]}

Reszka, Natalia ^{[1
]}

Lienlaf, Maritza ^{[1
]}

Arhel, Nathalie ^{[2
]}

Perez, Patricio ^{[1
]}

Brass, Abraham L. ^{[3
,4
]}

Diaz-Griffero, Felipe ^{[1
]}

机构：

[1] Albert Einstein Coll Med, Dept Microbiol & Immunol, Bronx, NY 10467 USA

[2] Inst Pasteur, Lab Virol Mol & Vaccinol, Paris, France

[3] Ragon Inst Massachusetts Gen Hosp MIT & Harvard U, Boston, MA USA

[4] Massachusetts Gen Hosp, Gastrointestinal Unit, Boston, MA 02114 USA

来源：

JOURNAL OF VIROLOGY | 2012年 / 86卷 / 10期

基金：

美国国家卫生研究院;

关键词：

DNA INTEGRATION; TRANSPORTIN-SR2; DOMAIN; VIRUS; PROTEINS;

D O I：

10.1128/JVI.00451-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

TNPO3 is a nuclear importer required for HIV-1 infection. Here, we show that depletion of TNPO3 leads to an HIV-1 block after nuclear import but prior to integration. To investigate the mechanistic requirement of TNPO3 in HIV-1 infection, we tested the binding of TNPO3 to the HIV-1 core and found that TNPO3 binds to the HIV-1 core. Overall, this work suggests that TNPO3 interacts with the incoming HIV-1 core in the cytoplasm to assist a process that is important for HIV-1 infection after nuclear import.

引用

页码：5931 / 5936

页数：6

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