Direct-current electrical field guides neuronal stem/progenitor cell migration

被引：121

作者：

Li, Lei ^{[2
,3
,4
]}

El-Hayek, Youssef H. ^{[2
]}

Liu, Baosong ^{[2
]}

Chen, Yonghong ^{[2
]}

Gomez, Everlyne ^{[2
]}

Wu, Xiaohua ^{[2
,3
,4
]}

Ning, Ke ^{[2
]}

Li, Lijun ^{[2
]}

Chang, Ning ^{[2
]}

Zhang, Liang ^{[2
]}

Wang, Zhengguo ^{[3
,4
]}

Hu, Xiang ^{[5
]}

Wan, Qi ^{[1
,2
]}

机构：

[1] Univ Nevada, Sch Med, Dept Physiol & Cell Biol, Reno, NV 89557 USA

[2] Univ Toronto, Toronto Western Res Inst, Univ Hlth Network, Div Fundamental Neurobiol, Toronto, ON, Canada

[3] Inst Surg Res, Chongqing, Peoples R China

[4] Daping Hosp, State Key Lab Trauma Burns & Combined Injury Chin, Chongqing, Peoples R China

[5] Beike Biotech Co Ltd, Shenzhen, Peoples R China

来源：

STEM CELLS | 2008年 / 26卷 / 08期

关键词：

direct-current electrical fields; neuronal stem/progenitor cells; migration; N-metilyl-D-aspartate receptor; GTPase;

D O I：

10.1634/stemcells.2007-1022

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Direct-current electrical fields (EFs) promote nerve growth and axon regeneration. We report here that at physiological strengths, EFs guide the migration of neuronal stem/progenitor cells (NSPCs) toward the cathode. F,F-directed NSPC migration requires activation of Nuriethyl-D-aspartate receptors (NMDARs), which leads to an increased physical association of Rho GTPase Ractassociated signals to the membrane NMDARs and the intracellular actin cytoskeleton. Thus, this study iden- tifies the EF as a directional guidance cue in controlling NSPC migration and reveals a role of the NMDAR/Racl/ actin signal transduction pathway in mediating EF-induced NSPC migration. These results suggest that as a safe physical approach in clinical application, EFs may be developed as a practical therapeutic strategy for brain repair by directing NSPC migration to the injured brain regions to replace cell loss.

引用

页码：2193 / 2200

页数：8

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