Rapid and sensitive method for the detection of Mycobacterium chlorophenolicum PCP-1 in soil based on 16S rRNA gene-targeted PCR

被引：15

作者：

Briglia, M ^{[1
]}

Eggen, RIL ^{[1
]}

deVos, WM ^{[1
]}

vanElsas, JD ^{[1
]}

机构：

[1] INST PLANT PROTECT,6700 GW WAGENINGEN,NETHERLANDS

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 04期

关键词：

D O I：

10.1128/AEM.62.4.1478-1480.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.

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收藏

页码：1478 / 1480

页数：3

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