Simultaneous determination of ritonavir and saquinavir, two human immunodeficiency virus protease inhibitors, in human serum by high-performance liquid chromatography

被引：45

作者：

Frappier, S

Breilh, D

Diarte, E

Ba, B

Ducint, D

Pellegrin, JL

Saux, MC

机构：

[1] Victor Segalen Univ Bordeaux, Clin Pharmacokinet Dept, F-33604 Pessac, France

[2] Pharm Haut Leveque Hosp, F-33604 Pessac, France

[3] Haut Leveque Hosp, Dept Infect Dis, F-33604 Pessac, France

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1998年 / 714卷 / 02期

关键词：

saquinavir; ritonavir;

D O I：

10.1016/S0378-4347(98)00220-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to describe an high-performance liquid chromatographic assay for the simultaneous determination of two HN protease inhibitors, saquinavir and ritonavir, in human serum. The method involved extraction of ritonavir and saquinavir from serum with the aid of solid-phase extraction C-18 cartridges followed by high-performance liquid chromatography with a C-8 column and ultraviolet detection set at a wavelength of 240 nm. The assay was linear and has been validated over the concentrations range of 0.5-32 mu g/ml for ritonavir and 0.075-4.8 mu g/ml for saquinavir, from 600 mu l serum extracted. In future, the assay will be used to support human population pharmacokinetic studies, and therapeutic drug monitoring for ritonavir and saquinavir. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：384 / 389

页数：6

共 12 条

[1] Nevirapine, zidovudine, and didanosine compared with zidovudine and didanosine in patients with HIV-1 infection - A randomized, double-blind, placebo-controlled trial [J].

DAquila, RT ;

Hughes, MD ;

Johnson, VA ;

Fischl, MA ;

Sommadossi, JP ;

Liou, SH ;

Timpone, J ;

Myers, M ;

Basgoz, N ;

Niu, M ;

Hirsch, MS ;

Costanzo, L ;

Ruben, S ;

Berzins, B ;

Martinez, A ;

Fishman, I ;

Kazial, K ;

Cort, SN ;

Robinson, P ;

Hall, D ;

Macy, H ;

McLaren, C ;

Rooney, J ;

Warwick, J ;

CavailleColl, M ;

Valentine, F ;

Booth, D ;

Soeiro, R ;

Stein, D ;

Zingman, B ;

Schliosberg, J ;

Polsky, B ;

Sepkowitz, K ;

Sharpe, V ;

Giordano, M ;

Wanke, C ;

Gulick, R ;

Craven, D ;

Grodman, C ;

Fife, K ;

Black, J ;

Todd, K ;

Nixon, H ;

Sperber, K ;

Gerits, P ;

Mildvan, D ;

Nicholas, P ;

Murphy, RL ;

Kessler, H ;

Pulvirenti, J .

ANNALS OF INTERNAL MEDICINE, 1996, 124 (12) :1019-1030

[2] ANTIVIRAL THERAPY FOR HUMAN-IMMUNODEFICIENCY-VIRUS INFECTIONS [J].

DECLERCQ, E .

CLINICAL MICROBIOLOGY REVIEWS, 1995, 8 (02) :200-239

[3]

GULICK R, 1996, 11 INT C AIDS VANC J

[4]

GULICK R, 1996, 3 C RETR OPP INF WAS

[5] Determination of saquinavir in human plasma by high-performance liquid chromatography [J].

Ha, HR ;

Follath, F ;

Bloemhard, Y ;

Krahenbuhl, S .

JOURNAL OF CHROMATOGRAPHY B, 1997, 694 (02) :427-433

[6] Determination of saquinavir in human plasma, saliva, and cerebrospinal fluid by ion-pair high-performance liquid chromatography with ultraviolet detection [J].

Hoetelmans, RMW ;

vanEssenberg, M ;

Meenhorst, PL ;

Mulder, JW ;

Beijnen, JH .

JOURNAL OF CHROMATOGRAPHY B, 1997, 698 (1-2) :235-241

[7]

HUBER L, 1998, LC GC INT FEB, P96

[8] QUANTIFICATION OF THE ANTI-HIV DRUG SAQUINAVIR BY HIGH-SPEED ONLINE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY TANDEM MASS-SPECTROMETRY [J].

KNEBEL, NG ;

SHARP, SR ;

MADIGAN, MJ .

JOURNAL OF MASS SPECTROMETRY, 1995, 30 (08) :1149-1156

[9] SELECTION AND ANALYSIS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-I VARIANTS WITH INCREASED RESISTANCE TO ABT-538, A NOVEL PROTEASE INHIBITOR [J].

MARKOWITZ, M ;

MO, HM ;

KEMPF, DJ ;

NORBECK, DW ;

BHAT, TN ;

ERICKSON, JW ;

HO, DD .

JOURNAL OF VIROLOGY, 1995, 69 (02) :701-706

[10] Current knowledge and future prospects for the use of HIV protease inhibitors [J].

Moyle, G ;

Gazzard, B .

DRUGS, 1996, 51 (05) :701-712

← 1 2 →