Styrene metabolism in Exophiala jeanselmei and involvement of a cytochrome P-450-dependent styrene monooxygenase

被引：51

作者：

Cox, HHJ ^{[1
]}

Faber, BW ^{[1
]}

VanHeiningen, WNM ^{[1
]}

Radhoe, H ^{[1
]}

Doddema, HJ ^{[1
]}

Harder, W ^{[1
]}

机构：

[1] TNO,INST ENVIRONM SCI ENERGY RES & PROC INNOVAT,DEPT ENVIRONM BIOTECHNOL,2600 JA DELFT,NETHERLANDS

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1996年 / 62卷 / 04期

关键词：

D O I：

10.1128/AEM.62.4.1471-1474.1996

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The yeast-like fungus Exophiala jeanselmei degrades styrene via initial oxidation of the vinyl side chain to phenylacetic acid, which is subsequently hydroxylated to homogentisic acid. The initial reactions are catalyzed by a NADPH- and flavin adenine dinucleotide-dependent styrene monooxygenase, a styrene oxide isomerase, and a NAD(+)-dependent phenylacetaldehyde dehydrogenase. The reduced GO-difference spectrum of microsomal preparations of styrene-grown cells shows a characteristic absorption maximum at 450 nm, which strongly suggests the involvement of a cytochrome P-450-dependent styrene monooxygenase. Inhibition of styrene monooxygenase activity in cell extracts by cytochrome P-450 inhibitors SKF-525-A, metyrapone, and CO confirms this assumption.

引用

页码：1471 / 1474

页数：4

共 26 条

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