The [FeFe] hydrogenase of Nyctotherus ovalis has a chimeric origin

被引：25

作者：

Boxma, Brigitte ^{[3
,9
]}

Ricard, Guenola ^{[1
,2
]}

van Hoek, Angela Ham ^{[3
,8
]}

Severing, Edouard ^{[3
]}

Moon-van der Staay, Seung-Yeo ^{[3
]}

van der Staay, Georg W. M. ^{[3
]}

van Alen, Theo A. ^{[3
]}

de Graaf, Rob M. ^{[3
]}

Cremers, Geert ^{[3
]}

Kwantes, Michiel ^{[3
]}

McEwan, Neil R. ^{[4
]}

Newbold, C. Jamie ^{[4
]}

Jouany, Jean-Pierre ^{[5
]}

Michalowski, Tadeusz ^{[6
]}

Pristas, Peter ^{[7
]}

Huynen, Martijn A. ^{[1
,2
]}

Hackstein, Johannes H. P. ^{[3
]}

机构：

[1] Radboud Univ Nijmegen, Nijmegen Med Ctr, CMBI 260, Ctr Mol & Biomol Informat, NL-6500 HB Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Nijmegen Med Ctr, CMBI 260, NCMLS, NL-6500 HB Nijmegen, Netherlands

[3] Radboud Univ Nijmegen, Fac Sci, Dept Evolut Microbiol, NL-6525 ED Nijmegen, Netherlands

[4] Aberystwyth Univ, Inst Rural Sci, Aberystwyth SY23 3AL, Ceredigion, Wales

[5] INRA, UR1213 Herbivores, F-63122 St Genes Champanelle, France

[6] Polish Acad Sci, Kielanowski Inst Anim Physiol & Nutr, PL-05110 Jablonna, Poland

[7] Slovak Acad Sci, Inst Anim Physiol, SK-04001 Kosice, Slovakia

[8] RIKILT, Inst Food Safety, Wageningen UR, Netherlands

[9] Intervet Int, Boxmeer, Netherlands

来源：

BMC EVOLUTIONARY BIOLOGY | 2007年 / 7卷 / 1期

关键词：

D O I：

10.1186/1471-2148-7-230

中图分类号：

Q [生物科学];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Background: The hydrogenosomes of the anaerobic ciliate Nyctotherus ovalis show how mitochondria can evolve into hydrogenosomes because they possess a mitochondrial genome and parts of an electron-transport chain on the one hand, and a hydrogenase on the other hand. The hydrogenase permits direct reoxidation of NADH because it consists of a [ FeFe] hydrogenase module that is fused to two modules, which are homologous to the 24 kDa and the 51 kDa subunits of a mitochondrial complex I. Results: The [ FeFe] hydrogenase belongs to a clade of hydrogenases that are different from well-known eukaryotic hydrogenases. The 24 kDa and the 51 kDa modules are most closely related to homologous modules that function in bacterial [ NiFe] hydrogenases. Paralogous, mitochondrial 24 kDa and 51 kDa modules function in the mitochondrial complex I in N. ovalis. The different hydrogenase modules have been fused to form a polyprotein that is targeted into the hydrogenosome. Conclusion: The hydrogenase and their associated modules have most likely been acquired by independent lateral gene transfer from different sources. This scenario for a concerted lateral gene transfer is in agreement with the evolution of the hydrogenosome from a genuine ciliate mitochondrion by evolutionary tinkering.

引用

页数：12

共 46 条

[1]

A hydrogenosome with a genome [J].