Detection of Cryptosporidium parvum in horses:: Thresholds of acid-fast stain, immunofluorescence assay, and flow cytometry

被引：22

作者：

Cole, DJ

Snowden, K

Cohen, ND

Smith, R

机构：

[1] Texas A&M Univ, Coll Vet Med, Dept Large Anim Med & Surg, College Stn, TX 77843 USA

[2] Texas A&M Univ, Coll Vet Med, Dept Vet Pathobiol, College Stn, TX 77843 USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 02期

关键词：

D O I：

10.1128/JCM.37.2.457-460.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10(1) to 10(6)/g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 x 10(5) oocysts/g of feces for the IFA and AF techniques and 5 x 10(4) oocysts/g for flow cytometry.

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页码：457 / 460

页数：4

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