Differential association of cytoplasmic signalling molecules SHP-1, SHP-2, SHIP and phospholipase C-γl with PECAM-1/CD31

被引：88

作者：

Pumphrey, NJ

Taylor, V

Freeman, S

Douglas, MR

Bradfield, PF

Young, SP

Lord, JM

Wakelam, MJO

Bird, IN

Salmon, M

Buckley, CD ^{[1
]}

机构：

[1] Univ Birmingham, Div Immun & Infect, Birmingham B15 2TT, W Midlands, England

[2] John Radcliffe Hosp, Inst Mol Med, Cell Adhes Lab, Oxford OX3 9DU, England

[3] Univ Birmingham, Div Canc Studies, Birmingham B15 2TT, W Midlands, England

[4] Univ Birmingham, MRC, Ctr Immune Regulat, Birmingham B15 2TT, W Midlands, England

[5] Yamanouchi Res Inst, Oxford OX4 4SX, England

来源：

FEBS LETTERS | 1999年 / 450卷 / 1-2期

基金：

英国惠康基金;

关键词：

platelet endothelial cell adhesion molecule 1; surface plasmon resonance; protein-protein interaction; Src homology 2 domain;

D O I：

10.1016/S0014-5793(99)00446-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recent studies have shown that, in addition to its role as an adhesion receptor, platelet endothelial cell adhesion molecule 1/CD31 becomes phosphorylated on tyrosine residues Y-663 and Y-686 and associates with protein tyrosine phosphatases SHP-1 and SHP-2, In this study, we screened for additional proteins which associate with phosphorylated platelet endothelial cell adhesion molecule 1, using surface plasmon resonance. We found that, besides SHP-1 and SHP-2, platelet endothelial cell adhesion molecule 1 binds the cytoplasmic signalling proteins SHIP and PLC-gamma 1 via their Src homology 2 domains. Using two phosphopeptides, NSDVQpY(663)TEVQV and DTETVpY(686)SEVRK, we demonstrate differential binding of SHP-1, SHP-2, SHIP and PLC-gamma 1. All four cytoplasmic signalling proteins directly associate with cellular platelet endothelial cell adhesion molecule 1, immunoprecipitated from pervanadate-stimulated THP-1 cells, These results suggest that overlapping immunoreceptor tyrosine-based inhibition motif/immunoreceptor tyrosine-based activation motif-like motifs within platelet endothelial cell adhesion molecule 1 mediate differential interactions between the Src homology 2 containing signalling proteins SHP-1, SHP-2, SHIP and PLC-gamma 1. (C) 1999 Federation of European Biochemical Societies.

引用

页码：77 / 83

页数：7

共 36 条

[1] Definition of the sites of interaction between the protein tyrosine phosphatase SHP-1 and CD22
Blasioli, J
Paust, S
Thomas, ML
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (04) : 2303 - 2307
[2] Inhibitory receptors abound?
Cambier, JC
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (12) : 5993 - 5995
[3] CAMBIER JC, 1995, J IMMUNOL, V155, P3281
[4] Regulation of mouse PECAM-1 tyrosine phosphorylation by the Src and Csk families of protein-tyrosine kinases
Cao, MY
Huber, M
Beauchemin, N
Famiglietti, J
Albelda, SM
Veillette, A
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (25) : 15765 - 15772
[5] Elias CG, 1998, EUR J IMMUNOL, V28, P1948, DOI 10.1002/(SICI)1521-4141(199806)28:06<1948::AID-IMMU1948>3.0.CO
[6] 2-C
[7] Tyrosine phosphorylation and relocation of SHIP are integrin-mediated in thrombin-stimulated human blood platelets
Giuriato, S
Payrastre, B
Drayer, AL
Plantavid, M
Woscholski, R
Parker, P
Erneux, C
Chap, H
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (43) : 26857 - 26863
[8] Engagement of human PECAM-1 (CD31) on human endothelial cells increases intracellular calcium ion concentration and stimulates prostacyclin release
Gurubhagavatula, I
Amrani, Y
Pratico, D
Ruberg, FL
Albelda, SM
Panettieri, RA
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1998, 101 (01) : 212 - 222
[9] Recruitment and activation of SHP-1 protein-tyrosine phosphatase by human platelet endothelial cell adhesion molecule-1 (PECAM-1) - Identification of immunoreceptor tyrosine-based inhibitory motif-like binding motifs and substrates
Hua, CT
Gamble, JR
Vadas, MA
Jackson, DE
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (43) : 28332 - 28340
[10] Characterization of phosphotyrosine binding motifs in the cytoplasmic domain of platelet endothelial cell adhesion molecule-1 (PECAM-1) that are required for the cellular association and activation of the protein-tyrosine phosphatase, SHP-2
Jackson, DE
Kupcho, KR
Newman, PJ
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (40) : 24868 - 24875

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