Cry3Aa11:: A new Cry3Aa δ-endotoxin from a local isolate of Bacillus thuringiensis

被引：12

作者：

Kurt, A

Özkan, M

Sezen, K

Demirbag, Z

Özcengiz, G

机构：

[1] Middle E Tech Univ, Dept Biol, TR-06531 Ankara, Turkey

[2] Gebze Inst High Technol, Dept Environm Engn, TR-41400 Kocaeli, Turkey

[3] Karadeniz Tech Univ, Dept Biol, TR-61080 Trabzon, Turkey

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 15期

关键词：

Bacillus thuringiensis; bioinsecticides; crystal protein; Cry3Aa delta-endotoxin; cry3Aa11;

D O I：

10.1007/s10529-005-8460-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A local isolate of Bacillus thuringiensis Mm2 had insecticidal activity against the larvae of Melolontha melolontha, Agelastica alni, Leptinotarsa decemlineata and Amphimallon solstitiale and produced a 65 kDa protein. SDS-PAGE profile of B. thuringiensis Mm2 was compared with those of 29 different Cry3Aa producers which verified Cry3Aa biosynthesis by the isolate. The cry3Aa gene of Mm2 was cloned, sequenced and the deduced amino acid sequence was compared with the cry3Aa sequences of ten different quaternary ranks. Its identity to these sequences ranged between 97.4% and 99.2%. The gene was next cloned into E. coli-Bacillus shuttle vector pNW33N and expressed at a low level in B. subtilis 168.

引用

页码：1117 / 1121

页数：5

共 10 条

[1] Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins [J].