17 beta-Estradiol metabolites affect some regulators of the MCF-7 cell cycle

被引：23

作者：

Lottering, ML ^{[1
]}

deKock, M ^{[1
]}

Viljoen, TC ^{[1
]}

Grobler, CJS ^{[1
]}

Seegers, JC ^{[1
]}

机构：

[1] MED UNIV S AFRICA, DEPT PHYSIOL, PRETORIA, SOUTH AFRICA

来源：

CANCER LETTERS | 1996年 / 110卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

PCNA; cdc(2); phosphorylation; 2-hydroxy-17; beta-estradiol; 2-methoxy-17;

D O I：

10.1016/S0304-3835(96)04489-8

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The activity of p34(cdc2) plays a key role in the regulation of the eukaryotic cell cycle. Another cell cycle associated molecule is PCNA. We investigated the effects of 2-hydroxy-17 beta-estradiol, a cell proliferator, and 2-methoxy-17 beta-estradiol, a potent inhibitor of cell growth, on the levels and activity of p34(cdc2) and on the levels of PCNA, as well as on protein phosphorylation in MCF-7 cells. 2-Hydroxyestradiol increased p34(cdc2) activity at G(I)/S and elevated PCNA levels during S-phase. 2-Methoxyestradiol caused unscheduled activation of p34(cdc2) in S-phase and decreased levels of p34(cdc2) and PCNA during G(2)/M. We conclude that 2-hydroxy- and 2-methoxyestradiol have definite, though different regulatory functions during the cell cycle.

引用

页码：181 / 186

页数：6

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