Contractile properties and proteins of smooth muscles of a calponin knockout mouse

被引:79
作者
Matthew, JD
Khromov, AS
McDuffie, MJ
Somlyo, AV
Somlyo, AP
Taniguchi, S
Takahashi, K
机构
[1] Univ Virginia, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22906 USA
[2] Univ Virginia, Dept Microbiol, Charlottesville, VA 22906 USA
[3] Shinshu Univ, Sch Med, Res Ctr Aging & Adaptat, Matsumoto, Nagano 3908621, Japan
[4] PRESTO, Osaka Med Ctr, Osaka 5378511, Japan
来源
JOURNAL OF PHYSIOLOGY-LONDON | 2000年 / 529卷 / 03期
关键词
D O I
10.1111/j.1469-7793.2000.00811.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. The role of h1-calponin in regulating the contractile properties of smooth muscle was investigated in bladder and vas deferens of mice carrying a targeted mutation in both alleles designed to inactivate the basic calponin gene. These calponin knockout (KO) mice displayed no detectable h1-calponin in their smooth muscles. 2. The amplitudes of Ca2+ sensitization, force and Ca2+ sensitivity were not significantly different in permeabilized smooth muscle of KO compared with wild-type (WT) mice, nor were the delays in onset and half-times of Ca2+ sensitization, initiated by flash photolysis of caged GTP gammaS, different. 3. The unloaded shortening velocity (V-us) of thiophosphorylated fibres was significantly (P < 0.05) faster in the smooth muscle of KO than WT animals, but could be slowed by exogenous calponin to approximate WT levels; the concentration dependence of exogenous calponin slowing of V-us was proportional to its actomyosin binding in situ. 4. Actin expression was reduced by 25-50%, relative to that of myosin heavy chain, in smooth muscle of KO mice, without any change in the relative distribution of the actin isoforms. 5. We conclude that the faster V-us of smooth muscle of the KO mouse is consistent with, but does not prove without further study, physiological regulation of the crossbridge cycle by calponin. Our results show no detectable role of calponin in the signal transduction of the Ca2+ sensitization pathways in smooth muscle.
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页码:811 / 824
页数:14
相关论文
共 56 条
[1]  
APPLEGATE D, 1994, J BIOL CHEM, V269, P10683
[2]   CONTRACTILE APPARATUS OF VASCULAR SMOOTH-MUSCLE - INTERMEDIATE HIGH-VOLTAGE STEREO ELECTRON-MICROSCOPY [J].
ASHTON, FT ;
SOMLYO, AV ;
SOMLYO, AP .
JOURNAL OF MOLECULAR BIOLOGY, 1975, 98 (01) :17-29
[3]   The Mouse Genome Database (MGD): expanding genetic and genomic resources for the laboratory mouse [J].
Blake, JA ;
Eppig, JT ;
Richardson, JE ;
Davisson, MT .
NUCLEIC ACIDS RESEARCH, 2000, 28 (01) :108-111
[4]  
BRETSCHER A, 1984, J BIOL CHEM, V259, P2873
[5]  
DIETRICH W, 1992, GENETICS, V131, P423
[6]   A comprehensive genetic map of the mouse genome [J].
Dietrich, WF ;
Miller, J ;
Steen, R ;
Merchant, MA ;
DamronBoles, D ;
Husain, Z ;
Dredge, R ;
Daly, MJ ;
Ingalls, KA ;
OConnor, TJ ;
Evans, CA ;
DeAngelis, MM ;
Levinson, DM ;
Kruglyak, L ;
Goodman, N ;
Copeland, NG ;
Jenkins, NA ;
Hawkins, TL ;
Stein, L ;
Page, DC ;
Lander, ES .
NATURE, 1996, 380 (6570) :149-152
[7]   NH2-terminal-inserted myosin II heavy chain is expressed in smooth muscle of small muscular arteries [J].
DiSanto, ME ;
Cox, RH ;
Wang, Z ;
Chacko, S .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 272 (05) :C1532-C1542
[8]   VELOCITY OF UNLOADED SHORTENING AND ITS RELATION TO SARCOMERE LENGTH AND ISOMETRIC FORCE IN VERTEBRATE MUSCLE-FIBERS [J].
EDMAN, KAP .
JOURNAL OF PHYSIOLOGY-LONDON, 1979, 291 (JUN) :143-159
[9]   FLASH-PHOTOLYSIS STUDIES OF RELAXATION AND CROSS-BRIDGE DETACHMENT - HIGHER SENSITIVITY OF TONIC THAN PHASIC SMOOTH-MUSCLE TO MGADP [J].
FUGLSANG, A ;
KHROMOV, A ;
TOROK, K ;
SOMLYO, AV ;
SOMLYO, AP .
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1993, 14 (06) :666-677
[10]   SMOOTH-MUSCLE SPECIFIC EXPRESSION OF CALPONIN [J].
GIMONA, M ;
HERZOG, M ;
VANDEKERCKHOVE, J ;
SMALL, JV .
FEBS LETTERS, 1990, 274 (1-2) :159-162