Contribution of nitric oxide to the depression of neuronal activity induced by temperature increase in the rat hippocampal CA1 area

Using optical recording techniques, we examined whether nitric oxide (NO) is implicated in the impairment of the activity of hippocampal CA1 neurons induced by mild heat stress. A temperature increase from 32 to 38 degreesC reversibly depressed the neuronal activity in hippocampal slices. L-Arginine (1 mM), an NO donor, enhanced the heat-induced depression of the activity of hippocampal CA1 neurons. N-omega-Nitro-L-arginine methyl ester, an inhibitor of nitric oxide synthase, attenuated the inhibition of the neuronal activity induced by a temperature increase. Methylcobalamin (10 muM), a vitamin B-12 analogue that reduces NO production, reduced the heat-induced depression of the neuronal activity. These results suggest that NO contributes, at least in part, to the heat-induced depression of the neuronal activity in the hippocampal CA1 region. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.