Glucosamine sulfate reduces experimental osteoarthritis and nociception in rats: association with changes of mitogen-activated protein kinase in chondrocytes

被引：83

作者：

Wen, Z-H ^{[1
]}

Tang, C. -C. ^{[2
]}

Chang, Y. -C. ^{[1
]}

Huang, S. -Y. ^{[1
]}

Hsieh, S. -P. ^{[3
]}

Lee, C. -H. ^{[4
]}

Huang, G. -S. ^{[5
]}

Ng, H-F ^{[6
]}

Neoh, C. -A. ^{[6
]}

Hsieh, C. -S. ^{[7
]}

Chen, W. -F. ^{[8
]}

Jean, Y. -H. ^{[9
]}

机构：

[1] Natl Sun Yat Sen Univ, Asia Pacific Ocean Res Ctr, Dept Marine Biotechnol & Resources, Kaohsiung 80424, Taiwan

[2] Natl Pingtung Univ Educ, Dept Early Childhood Educ, Pingtung, Taiwan

[3] Pingtung Christian Hosp, Sect Pathol, Pingtung City 900, Taiwan

[4] Taipei Med Univ Hosp, Dept Orthoped & Traumatol, Taipei, Taiwan

[5] Natl Def Med Ctr, Dept Radiol, Tri Serv Gen Hosp, Taipei, Taiwan

[6] Pingtung Christian Hosp, Sect Anesthesia, Pingtung City 900, Taiwan

[7] Pingtung Christian Hosp, Pediat Surg Sect, Dept Surg, Pingtung City 900, Taiwan

[8] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Neurosurg,Kaohsiung Med Ctr, Kaohsiung, Taiwan

[9] Pingtung Christian Hosp, Sect Orthoped Surg, Pingtung City 900, Taiwan

来源：

OSTEOARTHRITIS AND CARTILAGE | 2010年 / 18卷 / 09期

关键词：

Glucosamine; Osteoarthritis; Nociception; Anterior cruciate ligament; MAPK; NITRIC-OXIDE SYNTHASE; ARTICULAR-CARTILAGE; ORAL GLUCOSAMINE; SUBCHONDRAL BONE; DOUBLE-BLIND; JOINT PAIN; PROGRESSION; MODEL; INHIBITOR; ARTHRITIS;

D O I：

10.1016/j.joca.2010.05.012

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Objective: To study the effects of oral glucosamine sulfate on the development of osteoarthritis (OA) and to examine concomitant changes in the nociceptive behavior of rats. Methods: OA was induced in Wistar rats by anterior cruciate ligament transection (ACLT) of the right knee; the left knee was untreated. The OA + glucosamine group received oral glucosamine sulfate (250 mg/kg/day) in a 2-g wafer once a day for 10 consecutive weeks starting at week 5 after ACLT. The OA group was treated as above with 2-g wafers (placebo). The control group of nave rats received 2-g wafers only. The glucosamine alone group comprised nave rats receiving glucosamine sulfate only. Nociceptive behavior (mechanical allodynia and weight-bearing distribution of hind paws) during OA development was analyzed pre- and 3, 6, 9, 12, 15, and 18 weeks post-ACLT. Macroscopic and histologic studies were then performed on the cartilage and synovia. Immunohistochemical analysis was performed to examine the effect of glucosamine on expression of mitogen-activated protein kinases (MAPKs) in the articular cartilage chondrocytes. Results: OA rats receiving glucosamine showed a significantly lower degree of cartilage degeneration than the rats receiving placebo. Glucosamine treatment also suppressed synovitis. Mechanical allodynia and weight-bearing distribution studies showed significant improvement in the OA + glucosamine group as compared to the OA group. Moreover, glucosamine attenuated p38 and c-Jun N-terminal kinase (JNK) but increased extracellular signal-regulated kinase 1/2 (ERK) expression in OA-affected cartilage. Conclusion: Our results indicate that treatment with oral glucosamine sulfate in a rat OA model (1) attenuates the development of OA, (2) concomitantly reduces nociception, and (3) modulates chondrocyte metabolism, possibly through inhibition of cell p38 and JNK and increase of ERK expression. (C) 2010 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.

引用

页码：1192 / 1202

页数：11

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