In Leymus chinensis, mild water stress (soil moisture 60-65% of field capacity) had no significant effects on nitrogen metabolism, photosynthesis, and chlorophyll fluorescence. Severe water stress (35-40%) significantly decreased the activities of nitrate reductase, glutamine synthetase, and glutamate dehydrogenase, net photosynthetic rate, stomatal conductance, transpiration rate, maximal efficiency of photosystem 2 photochemistry (F-v/F-m), actual quantum yield, and photochemical quenching, but increased the endopeptidase activity and malondialdehyde contents. The adverse effects on photosynthesis and N metabolism were markedly greater in reproductive shoots than in vegetative shoots.