Tissue transglutaminase expression affects hypusine metabolism in BALB/c 3T3 cells

被引：21

作者：

Beninati, S

Gentile, V

Caraglia, M

Lentini, A

Tagliaferri, P

Abbruzzese, A

机构：

[1] Univ Naples 2, Dept Biochem & Biophys, I-80132 Naples, Italy

[2] Univ Roma Tor Vergata 2, Dept Biol, I-00173 Rome, Italy

[3] Univ Naples Federico II, Dept Mol & Clin Endocrinol & Oncol, Naples, Italy

来源：

FEBS LETTERS | 1998年 / 437卷 / 1-2期

关键词：

transglutaminase; eukaryotic initiation factor 5A; hypusine; polyamine; post-translational modification;

D O I：

10.1016/S0014-5793(98)01191-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Post-translational formation of hypusine in eukaryotic initiation factor 5A (eIF-5A) is essential for cell viability. Recently, we showed that hypusine protein is an in vitro substrate for transglutaminases (TGases), We report the effect of tissue TGase expression on the in vivo hypusine metabolic pathway. The stable expression of tTGase in BALB/c 3T3 cells induced a 100-fold reduction of hypusine levels and a 50% increase of gamma-glutamyl- <(omega)over bar>-hypusine formation. Such changes mere paralleled by a consistent decrease in the free polyamine pool and an enhancement of their excretion and of the formation of their gamma- glutamyl derivatives, These effects occurred together with a significant reduction of cell proliferation, in this report we suggest, for the first time, that tTGase affects hypusine metabolism, thus regulating the eIF-5A activity and cell proliferation. (C) 1998 Federation of European Biochemical Societies.

引用

页码：34 / 38

页数：5

共 30 条

[1] DEVELOPMENTAL PATTERN FOR DEOXYHYPUSINE HYDROXYLASE IN RAT-BRAIN [J].