Generating somatic mosaicism with a Cre recombinase - microsatellite sequence transgene

被引：30

作者：

Akyol, Aytekin ^{[1
]}

Hinoi, Takao ^{[1
,5
]}

Feng, Ying ^{[1
]}

Bommer, Guido T. ^{[1
]}

Glaser, Thomas M. ^{[1
,2
]}

Fearon, Eric R. ^{[1
,3
,4
]}

机构：

[1] Univ Michigan, Sch Med, Dept Internal Med, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Sch Med, Dept Human Genet, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Sch Med, Ctr Canc, Ann Arbor, MI 48109 USA

[5] Hiroshima Univ, Dept Surg, Minami Ku, Hiroshima 7348551, Japan

来源：

NATURE METHODS | 2008年 / 5卷 / 03期

关键词：

D O I：

10.1038/NMETH.1182

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Strategies for altering constitutional or somatic genotype in mice are well established, but approaches to generate mosaic genotypes in mouse tissues are limited. We showed that a functionally inactive Cre recombinase transgene with a long mononucleotide tract altering the reading frame was stochastically activated in the mouse intestinal tract. We demonstrated the utility of this approach by inducing colonic polyposis after Cre-mediated bi-allelic inactivation of the Apc gene.

引用

收藏

页码：231 / 233

页数：3

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