Direct interaction between metastasis-associated protein 1 and endophilin 3

被引：23

作者：

Aramaki, Y

Ogawa, K

Toh, Y

Ito, T

Akimitsu, N

Hamamoto, H

Sekimizu, K

Matsusue, K

Kono, A

Iguchi, H

Takiguchi, S

机构：

[1] Kyushu Natl Canc Ctr, Inst Clin Res, Minami Ku, Fukuoka 8111395, Japan

[2] Kyushu Natl Canc Ctr, Dept Surg Gastroenterol, Minami Ku, Fukuoka 8111395, Japan

[3] Univ Tokyo, Grad Sch Pharmaceut Sci, Dept Dev Biochem, Bunkyo Ku, Tokyo 1130033, Japan

[4] Fukuoka Univ, Fac Pharmaceut Sci, Dept Hyg Chem, Fukuoka 8140180, Japan

来源：

FEBS LETTERS | 2005年 / 579卷 / 17期

关键词：

endocytosis; yeast two-hybrid system; cancer metastasis; NuRD complex; histone deacetylase; chromatin; estrogen receptor;

D O I：

10.1016/j.febslet.2005.05.069

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The yeast two-hybrid system was used to search for partners of mouse metastasis-associated protein 1 (Mta1). Screening of a cDNA library prepared from mouse embryo yielded positive clones coding for endophilin 3. The site of interaction was suggested to be the SH-3-binding domain of Mta1 and SH-3 domain of endophilin 3. This interaction was confirmed by GST pull-down assay in vitro and immunoprecipitation in vivo. The Mta1 and endophilin 3 transcripts were highly expressed in testis and brain. But, Mta1 localized mainly in nucleus and to a lesser extent in cytoplasm while endophilin 3 localized mainly in cytoplasm. If Mta1 functions in cytoplasm, it might be involved in the regulation of endocytosis mediated by endophilin 3. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3731 / 3736

页数：6

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