Serological Studies Confirm the Novel Astrovirus HMOAstV-C as a Highly Prevalent Human Infectious Agent

被引:52
作者
Burbelo, Peter D. [1 ]
Ching, Kathryn H. [1 ]
Esper, Frank [2 ]
Iadarola, Michael J. [1 ]
Delwart, Eric [3 ,4 ]
Lipkin, W. Ian [5 ]
Kapoor, Amit [5 ]
机构
[1] Natl Inst Dent & Craniofacial Res, Neurobiol & Pain Therapeut Sect, Lab Sensory Biol, NIH, Bethesda, MD USA
[2] Case Western Reserve Univ Hosp, Med Ctr, Dept Pediat, Cleveland, OH 44106 USA
[3] Univ Calif San Francisco, Blood Syst Res Inst, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA
[5] Columbia Univ, Ctr Infect & Immun, New York, NY USA
来源
PLOS ONE | 2011年 / 6卷 / 08期
关键词
LUCIFERASE IMMUNOPRECIPITATION SYSTEMS; HUMAN STOOL; CHILDREN; DIARRHEA; GASTROENTERITIS; IDENTIFICATION; DIAGNOSIS; VIRUS; ASSAY; SEQUENCES;
D O I
10.1371/journal.pone.0022576
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Molecular identification of a microbe is the first step in determining its prevalence of infection and pathogenic potential. Detection of specific adaptive immune responses can provide insights into whether a microbe is a human infectious agent and its epidemiology. Here we characterized human anti-IgG antibody responses by luciferase immunoprecipitation systems (LIPS) against two protein fragments derived from the capsid protein of the novel HMOAstV-C astrovirus. While antibodies to the N-terminal fragment were not informative, the C-terminal capsid fragment of HMOAstV-C showed a high frequency of immunoreactivity with serum from healthy blood donors. In contrast, a similar C-terminal capsid fragment from the related HMOAstV-A astrovirus failed to show immunoreactivity. Detailed analysis of adult serum from the United Sates using a standardized threshold demonstrated HMOAstV-C seropositivity in approximately 65% of the samples. Evaluation of serum samples from different pediatric age groups revealed that the prevalence of antibodies in 6-12 month, 1-2 year, 2-5 year and 5-10 year olds was 20%, 23%, 32% and 36%, respectively, indicating rising seroprevalence with age. Additionally, 50% (11/22) of the 0-6 month old children showed anti-HMOAstV-C antibody responses, likely reflecting maternal antibodies. Together these results document human humoral responses to HMOAstV-C and validate LIPS as a facile and effective approach for identifying humoral responses to novel infectious agents.
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页数:5
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