Analyses of secondary structures in DNA by pyrosequencing

被引：63

作者：

Ronaghi, M ^{[1
]}

Nygren, M ^{[1
]}

Lundeberg, J ^{[1
]}

Nyrén, P ^{[1
]}

机构：

[1] Royal Melbourne Inst Technol, Dept Biotechnol, SE-10044 Stockholm, Sweden

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 267卷 / 01期

关键词：

nucleic acids; compression; secondary structures; DNA sequencing; pyrosequencing; inorganic pyrophosphate; nucleotide degradation; apyrase; bioassay;

D O I：

10.1006/abio.1998.2978

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A common problem in conventional DNA sequencing is the occurrence of DNA sequence compressions during gel electrophoresis, leading to misreading of the sequence. These compressions are usually due to secondary structures in the DNA fragment. In this study, we present a non-gel-based DNA sequencing technique that facilitates analysis of such DNA regions. A part of the polymorphic pertussis toxin promoter region in five different Bordetella species was successfully resolved by the new technique. The obtained sequence data revealed four related palindromic sequences. The ability of different DNA polymerases to read through such secondary structures is also described, (C) 1999 Academic Press.

引用

页码：65 / 71

页数：7

共 31 条

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