The Basic Helix-Loop-Helix Transcription Factor MYC2 Directly Represses PLETHORA Expression during Jasmonate-Mediated Modulation of the Root Stem Cell Niche in Arabidopsis

被引:388
作者
Chen, Qian [1 ]
Sun, Jiaqiang [1 ,2 ]
Zhai, Qingzhe [1 ,2 ]
Zhou, Wenkun [1 ]
Qi, Linlin [1 ]
Xu, Li [1 ]
Wang, Bao [1 ]
Chen, Rong [1 ]
Jiang, Hongling [1 ,2 ]
Qi, Jing [2 ,3 ,4 ]
Li, Xugang [2 ,3 ,4 ]
Palme, Klaus [2 ,3 ,4 ]
Li, Chuanyou [1 ,2 ]
机构
[1] Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res, State Key Lab Plant Genom, Beijing 100101, Peoples R China
[2] Chinese Acad Sci, Inst Genet & Dev Biol, Chinese German Joint Grp Plant Hormone Res, Beijing 100101, Peoples R China
[3] Univ Freiburg, Fac Biol, Inst Biol Bot 2, D-79104 Freiburg, Germany
[4] Univ Freiburg, Fac Biol, Freiburg Inst Adv Sci, D-79104 Freiburg, Germany
基金
中国国家自然科学基金;
关键词
ETHYLENE-INSENSITIVE MUTANTS; BOX PROTEIN TIR1; AUXIN BIOSYNTHESIS; METHYL JASMONATE; PLANT-GROWTH; REGULATED DEFENSE; TRANSPORT; SIGNAL; DIFFERENTIATION; RESISTANCE;
D O I
10.1105/tpc.111.089870
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The root stem cell niche, which in the Arabidopsis thaliana root meristem is an area of four mitotically inactive quiescent cells (QCs) and the surrounding mitotically active stem cells, is critical for root development and growth. We report here that during jasmonate-induced inhibition of primary root growth, jasmonate reduces root meristem activity and leads to irregular QC division and columella stem cell differentiation. Consistently, jasmonate reduces the expression levels of the AP2-domain transcription factors PLETHORA1 (PLT1) and PLT2, which form a developmentally instructive protein gradient and mediate auxin-induced regulation of stem cell niche maintenance. Not surprisingly, the effects of jasmonate on root stem cell niche maintenance and PLT expression require the functioning of MYC2/JASMONATE INSENSITIVE1, a basic helixloop-helix transcription factor that involves versatile aspects of jasmonate-regulated gene expression. Gel shift and chromatin immunoprecipitation experiments reveal that MYC2 directly binds the promoters of PLT1 and PLT2 and represses their expression. We propose that MYC2-mediated repression of PLT expression integrates jasmonate action into the auxin pathway in regulating root meristem activity and stem cell niche maintenance. This study illustrates a molecular framework for jasmonate-induced inhibition of root growth through interaction with the growth regulator auxin.
引用
收藏
页码:3335 / 3352
页数:18
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