New insights into the epidemiology of bovine piroplasmoses in Italy

被引:21
作者
Cassini, R. [1 ]
Marcer, F. [1 ]
di Regalbono, A. Frangipane [1 ]
Cancrini, G. [2 ]
Gabrielli, S. [2 ]
Moretti, A. [3 ]
Galuppi, R. [4 ]
Tampieri, M. P. [4 ]
Pietrobelli, M. [1 ]
机构
[1] Univ Padua, Dipartimento Sci Sperimentali Vet, I-35020 Legnaro, PD, Italy
[2] Univ Roma La Sapienza, Dipartimento Sanita Pubbl & Malattie Infett, I-00185 Rome, Italy
[3] Univ Perugia, Dipartimento Sci Biopatol & Igiene Prod Anim & Al, I-06126 Perugia, Italy
[4] Alma Mater Studiorum Univ Bologna, Dipartimento Sanita Pubbl Vet & Patol Anim, I-40064 Ozzano Dell Emilia, BO, Italy
关键词
Babesia spp; Theileria spp; Ticks; Bovine; Italy; BABESIA-DIVERGENS; 1ST REPORT; THEILERIA; CATTLE; HEPATOZOON; DISEASES; BIGEMINA; ANIMALS;
D O I
10.1016/j.vetpar.2011.08.004
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
090105 [作物生产系统与生态工程]; 100103 [病原生物学];
摘要
Few studies have been published on bovine piroplasmoses in Italy, and therefore a clear picture of the epidemiology of these infections is difficult to obtain. Vertebrate and invertebrate hosts in Central and Northern Regions of Italy were investigated in 2005 and 2006, when microscopy, molecular tools and serological tests were applied to 468 blood samples drawn from cattle in order to evaluate the presence of these protozoa and identify possible risk factors. Ticks were also collected, identified and analyzed by molecular techniques. Microscopy identified 6.5% of the animals as positive, whereas PCR detected piroplasm DNA in 21.6%. BLAST analysis showed 67 amplicons (17.0%) referable to the Theileria sergenti/buffeli/orientalis group, 17(4.3%) to Theileria annae, and Ito Babesia divergens. Serology evidenced a prevalence of 45.4% for Babesia bovis, 17.4% for Babesia bigemina, and 34.9% for B. divergens. The 127 collected ticks were identified as belonging to 5 species, mostly represented by Rhipicephalus bursa, Hyalomma marginatum and Ixodes ricin us. Molecular analyses evidenced the presence of B. bovis and B. bigemina, in 3 and 5 ticks, respectively. Our findings suggest that different species of piroplasms are circulating in bovine populations in Central and Northern Italy, and provide new insights into the complex epidemiology of bovine piroplasmoses in Italy. (C) 2011 Elsevier B.V. All rights reserved.
引用
收藏
页码:77 / 82
页数:6
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