Rapid detection of methicillin resistance in coagulase-negative staphylococci by a penicillin-binding protein 2A-specific latex agglutination test

被引：19

作者：

Horstkotte, MA ^{[1
]}

Knobloch, JKM ^{[1
]}

Rohde, H ^{[1
]}

Mack, D ^{[1
]}

机构：

[1] Univ Hamburg, Klinikum Eppendorf, Inst Med Mikrobiol & Immunol, D-20246 Hamburg, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2001年 / 39卷 / 10期

关键词：

D O I：

10.1128/JCM.39.10.3700-3702.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The detection of PBP 2a by the MRSA-Screen latex agglutination test with 201 clinical coagulase-negative staphylococci had an initial sensitivity of 98% and a high degree of specificity for Staphylococcus epidermidis strains compared to PCR for mecA. Determination of oxacillin MICs evaluated according to the new breakpoint (0.5 mug/ml) of the National Committee for Clinical Laboratory Standards exhibited an extremely low specificity for this population.

引用

页码：3700 / 3702

页数：3

共 28 条

[1] Establishing MIC breakpoints for coagulase-negative staphylococci to oxacillin [J].

Andrews, JM ;

Boswell, FJ ;

Wise, R .

JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 45 (02) :259-261

[2]

[Anonymous], STAPHYLOCOCCI HUMAN

[3] ANTIMICROBIAL SUSCEPTIBILITY OF COAGULASE-NEGATIVE STAPHYLOCOCCI [J].

ARCHER, GL ;

CLIMO, MW .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (10) :2231-2237

[4] Evaluation of MRSA-screen, a simple anti-PBP 2a slide latex agglutination kit, for rapid detection of methicillin resistance in Staphylococcus aureus [J].

Cavassini, M ;

Wenger, A ;

Jaton, K ;

Blanc, DS ;

Bille, J .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (05) :1591-1594

[5]

Cavassini M, 1999, J CLIN MICROBIOL, V37, P3784

[6] Methicillin resistance in staphylococci: Molecular and biochemical basis and clinical implications [J].

Chambers, HF .

CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (04) :781-+

[7]

Favero MS, 1996, AM J INFECT CONTROL, V24, P380

[8] Correlation of oxacillin MIC with mecA gene carriage in coagulase-negative staphylococci [J].

Hussain, Z ;

Stoakes, L ;

Massey, V ;

Diagre, D ;

Fitzgerald, V ;

El Sayed, S ;

Lannigan, R .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (02) :752-754

[9]

Hussain Z, 2000, J CLIN MICROBIOL, V38, P2051

[10]

Jorgenson J. H., 1999, MANUAL CLIN MICROBIO, P1526

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