Fate of mat1 DNA strands during mating-type switching in fission yeast

被引:26
作者
Arcangioli, B [1 ]
机构
[1] Inst Pasteur, Dept Biotechnol, CNRS, URA 1644,Unite Virus Oncogenes, F-75724 Paris 15, France
关键词
D O I
10.1093/embo-reports/kvd023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The mating-type switching of the fission yeast, Schizosaccharomyces pombe, is highly regulated. Two consecutive asymmetric divisions are required to produce one mating-type switched cell among the four progeny. Using DNA density-gradient centrifugation we demonstrate that one-fourth of the mat1 DNA is not replicated by the conventional semi-conservative mode, but instead both DNA strands are synthesized denovo. Our data are consistent with a gene conversion event, initiated by a site- and strand-specific DNA break (SSB). We further demonstrate that the virgin switched mat1-containing chromatid no longer contained the nick, while it is reintroduced during the lagging strand synthesis of the mat1 locus on the sister chromatid. This finding establishes at the molecular level a firm experimental link between the phenotype and genotype in the process of asymmetric mating-type switching during mitotic divisions.
引用
收藏
页码:145 / 150
页数:6
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