Imaging Drosophila gene activation and polymerase pausing in vivo

被引：56

作者：

Lis, John T. ^{[1
]}

机构：

[1] Cornell Univ, Ithaca, NY 14853 USA

来源：

NATURE | 2007年 / 450卷 / 7167期

关键词：

D O I：

10.1038/nature06324

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Since the early 1960s, imaging studies of Drosophila sp. polytene chromosomes have provided unique views of gene transcription in vivo. The dramatic changes in chromatin structure that accompany gene activation can be visualized as chromosome puffs. Now, live-cell imaging techniques coupled with protein-DNA crosslinking assays on a genome-wide scale allow more detailed mechanistic questions to be addressed and are prompting the re-evaluation of models of transcription regulation in both Drosophila and mammals.

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页码：198 / 202

页数：5

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