Use of methanolysis for the determination of total ellagic and gallic acid contents of wood and food products

被引:67
作者
Lei, Z [1 ]
Jervis, J [1 ]
Helm, RF [1 ]
机构
[1] Virginia Polytech Inst & State Univ, Dept Wood Sci & Forest Prod, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA
关键词
ellagic acid; gallic acid; HPLC; ellagitannins; methanolysis; methyl gallate;
D O I
10.1021/jf000974a
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Anhydrous methanolic HCl has been found to be an excellent reagent for releasing ellagic acid and gallic acid (as methyl gallate) from biomass substrates. Optimization of both the reaction conditions and the gradient HPLC analysis has led to the development of a new protocol. The method provides ellagic acid yields significantly higher than those obtained previously, indicating total ellagic acid contents of several substrates have previously been underestimated.
引用
收藏
页码:1165 / 1168
页数:4
相关论文
共 29 条
[1]  
ANCOS DB, 2000, J AGR FOOD CHEM, V48, P4565
[2]   Quantitative analysis of ellagic acid in hardwood samples [J].
Bianco, MA ;
Handaji, A ;
Savolainen, H .
SCIENCE OF THE TOTAL ENVIRONMENT, 1998, 222 (1-2) :123-126
[3]  
Clifford MN, 2000, J SCI FOOD AGR, V80, P1118, DOI [10.1002/(SICI)1097-0010(20000515)80:7&lt
[4]  
1118::AID-JSFA570&gt
[5]  
3.0.CO
[6]  
2-9, 10.1002/(SICI)1097-0010(20000515)80:7<1118::AID-JSFA570>3.0.CO
[7]  
2-9]
[8]  
DANIEL E M, 1989, Journal of Food Composition and Analysis, V2, P338, DOI 10.1016/0889-1575(89)90005-7
[9]  
FIESER LF, 1967, REAGENTS ORGANIC SYN, V1, P668
[10]  
Häkkinen SH, 1998, J SCI FOOD AGR, V77, P543, DOI [10.1002/(SICI)1097-0010(199808)77:4<543::AID-JSFA78>3.0.CO