A monoclonal antibody for G protein-coupled receptor crystallography

被引：90

作者：

Day, Peter W.

Rasmussen, Soren G. F.

Parnot, Charles

Fung, Juan Jose

Masood, Asna

Kobilka, Tong Sun

Yao, Xiao-Jie

Choi, Hee-Jung

Weis, William I.

Rohrer, Daniel K.

Kobilka, Brian K.

机构：

[1] Stanford Univ, Sch Med, Dept Cellular & Mol Physiol, Stanford, CA 94305 USA

[2] Medarex Inc, Milpitas, CA 95035 USA

[3] Stanford Univ, Sch Med, Dept Biol Struct, Palo Alto, CA 94305 USA

来源：

NATURE METHODS | 2007年 / 4卷 / 11期

关键词：

D O I：

10.1038/NMETH1112

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

G protein-coupled receptors (GPCRs) constitute the largest family of signaling proteins in mammals, mediating responses to hormones, neurotransmitters, and senses of sight, smell and taste. Mechanistic insight into GPCR signal transduction is limited by a paucity of high-resolution structural information. We describe the generation of a monoclonal antibody that recognizes the third intracellular loop (IL3) of the native human beta(2) adrenergic (b2AR) receptor; this antibody was critical for acquiring diffraction-quality crystals.

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页码：927 / 929

页数：3

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