A chicory cytochrome P450 mono-oxygenase CYP71AV8 for the oxidation of (+)-valencene

被引：90

作者：

Cankar, Katarina ^{[1
]}

van Houwelingen, Adele

Bosch, Dirk

Sonke, Theo ^{[2
]}

Bouwmeester, Harro ^{[1
]}

Beekwilder, Jules ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Lab Plant Physiol, NL-6708 PB Wageningen, Netherlands

[2] Isobionics, NL-6167 RD Geleen, Netherlands

来源：

FEBS LETTERS | 2011年 / 585卷 / 01期

关键词：

Cytochrome P450; Sesquiterpene; Valencene; Germacrene A; Amorpha-4,11-diene; Regio-specificity; Cichorium intybus; FUNCTIONAL-CHARACTERIZATION; YEAST EXPRESSION; NOOTKATONE; SESQUITERPENES; BIOSYNTHESIS; VALENCENE; SYNTHASE; CLONING;

D O I：

10.1016/j.febslet.2010.11.040

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Chicory (Cichorium intybus L.), which is known to have a variety of terpene-hydroxylating activities, was screened for a P450 mono-oxygenase to convert (+)-valencene to (+)-nootkatone. A novel P450 cDNA was identified in a chicory root EST library. Co-expression of the enzyme with a valencene synthase in yeast, led to formation of trans-nootkatol, cis-nootkatol and (+)-nootkatone. The novel enzyme was also found to catalyse a three step conversion of germacrene A to germacra-1(10), 4,11(13)-trien-12-oic acid, indicating its involvement in chicory sesquiterpene lactone biosynthesis. Likewise, amorpha-4,11-diene was converted to artemisinic acid. Surprisingly, the chicory P450 has a different regio-specificity on (+)-valencene compared to germacrene A and amorpha-4,11-diene. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.

引用

页码：178 / 182

页数：5

共 22 条

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