Transmitters and Pathways Mediating Inhibition of Spinal Itch-Signaling Neurons by Scratching and Other Counterstimuli

被引:85
作者
Akiyama, Tasuku [1 ]
Carstens, Mirela Iodi [1 ]
Carstens, Earl [1 ]
机构
[1] Univ Calif Davis, Dept Neurobiol Physiol & Behav, Davis, CA 95616 USA
来源
PLOS ONE | 2011年 / 6卷 / 07期
基金
美国国家卫生研究院;
关键词
DORSAL-HORN NEURONS; HISTAMINE-INDUCED ITCH; DRY SKIN; PAR-2; AGONIST; ACTIVATED RECEPTORS; SENSORY NEURONS; FOS EXPRESSION; POTENTIAL ROLE; PAIN; MOUSE;
D O I
10.1371/journal.pone.0022665
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Scratching relieves itch, but the underlying neural mechanisms are poorly understood. We presently investigated a role for the inhibitory neurotransmitters GABA and glycine in scratch-evoked inhibition of spinal itch-signaling neurons in a mouse model of chronic dry skin itch. Superficial dorsal horn neurons ipsilateral to hindpaw dry skin treatment exhibited a high level of spontaneous firing that was significantly attenuated by cutaneous scratching, pinch and noxious heat. Scratch-evoked inhibition was nearly abolished by spinal delivery of the glycine antagonist, strychnine, and was markedly attenuated by respective GABAA and GABAB antagonists bicuculline and saclofen. Scratch-evoked inhibition was also significantly attenuated (but not abolished) by interruption of the upper cervical spinal cord, indicating the involvement of both segmental and suprasegmental circuits that engage glycine- and GABA-mediated inhibition of spinal itch-signaling neurons by noxious counterstimuli.
引用
收藏
页数:10
相关论文
共 40 条
[1]   Enhanced responses of lumbar superficial dorsal horn neurons to intradermal PAR-2 agonist but not histamine in a mouse hindpaw dry skin itch model [J].
Akiyama, Tasuku ;
Carstens, Mirela Iodi ;
Carstens, E. .
JOURNAL OF NEUROPHYSIOLOGY, 2011, 105 (06) :2811-2817
[2]   Spontaneous itch in the absence of hyperalgesia in a mouse hindpaw dry skin model [J].
Akiyama, Tasuku ;
Carstens, Mirela Iodi ;
Carstens, E. .
NEUROSCIENCE LETTERS, 2010, 484 (01) :62-65
[3]   Excitation of Mouse Superficial Dorsal Horn Neurons by Histamine and/or PAR-2 Agonist: Potential Role in Itch [J].
Akiyama, Tasuku ;
Carstens, Mirela Iodi ;
Carstens, E. .
JOURNAL OF NEUROPHYSIOLOGY, 2009, 102 (04) :2176-2183
[4]   Scratching Behavior and Fos Expression in Superficial Dorsal Horn Elicited by Protease-Activated Receptor Agonists and Other Itch Mediators in Mice [J].
Akiyama, Tasuku ;
Merrill, Austin W. ;
Zanotto, Karen ;
Carstens, Mirela Iodi ;
Carstens, E. .
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2009, 329 (03) :945-951
[5]   Activation of Superficial Dorsal Horn Neurons in the Mouse by a PAR-2 Agonist and 5-HT: Potential Role in Itch [J].
Akiyama, Tasuku ;
Merrill, Austin W. ;
Carstens, Mirela Iodi ;
Carstens, E. .
JOURNAL OF NEUROSCIENCE, 2009, 29 (20) :6691-6699
[6]  
Bickford RG, 1938, CLIN SCI, V3, P377
[7]   EFFECTS OF MENTHOL AND COLD ON HISTAMINE-INDUCED ITCH AND SKIN REACTIONS IN MAN [J].
BROMM, B ;
SCHAREIN, E ;
DARSOW, U ;
RING, J .
NEUROSCIENCE LETTERS, 1995, 187 (03) :157-160
[8]  
CARSTENS E, 2009, CURRENT TOPICS PAIN, P79
[9]   Dorsal horn neurons expressing NK-1 receptors mediate scratching in rats [J].
Carstens, Earl E. ;
Carstens, Mirela Iodi ;
Simons, Christopher T. ;
Jinks, Steven L. .
NEUROREPORT, 2010, 21 (04) :303-308
[10]   Junctional versus extrajunctional glycine and GABAA receptor-mediated IPSCs in identified lamina I neurons of the adult rat spinal cord [J].
Chéry, N ;
De Koninck, Y .
JOURNAL OF NEUROSCIENCE, 1999, 19 (17) :7342-7355