ELISA test for anti-neutrophil cytoplasm antibodies detection evaluated by a computer screen photo-assisted technique

被引:9
作者
Filippini, D [1 ]
Tejle, K
Lundström, I
机构
[1] Linkoping Univ, Div Appl Phys, Inst Phys & Measurement Technol, S-58183 Linkoping, Sweden
[2] Linkoping Univ, Dept Mol & Clin Med, Div Med Microbiol, Fac Hlth Sci, S-58185 Linkoping, Sweden
关键词
computer screen photo-assisted technique; bioassays; ELISA; medical diagnostics; home tests; point of care;
D O I
10.1016/j.bios.2004.09.033
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The computer screen photo-assisted technique (CSPT), a method for substance classification based on spectral fingerprinting, which involves just a computer screen and a web camera as measuring platform is used here for the evaluation of a prospective enzyme-linked immunosorbent assay (ELISA). A anti-neutrophil cytoplasm antibodies (ANCA-ELISA) test, typically used for diagnosing patients suffering from chronic inflammatory disorders in the skin, joints, blood vessels and other tissues is comparatively tested with a standard microplate reader and CSPT, yielding equivalent results at a fraction of the instrumental costs. The CSPT approach is discussed as a distributed measuring platform allowing decentralized measurements in routine applications, whereas keeping centralized information management due to its natural network embedded operation. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:266 / 272
页数:7
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