Comprehensive DNA microarray analysis of Bacillus subtilis two-component regulatory systems

被引:118
作者
Kobayashi, K
Ogura, M
Yamaguchi, H
Yoshida, KI
Ogasawara, N
Tanaka, T
Fujita, Y
机构
[1] Fukuyama Univ, Fac Engn, Dept Biotechnol, Hiroshima 7290292, Japan
[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300101, Japan
[3] Tokai Univ, Dept Marine Sci & Technol, Shimizu, Shizuoka 4248610, Japan
关键词
D O I
10.1128/JB.183.24.7365-7370.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
It has recently been shown through DNA microarray analysis of Bacillus subtilis two-component regulatory systems (DegS-DegU, ComP-ComA, and PhoR-PhoP) that overproduction of a response regulator of the two-component systems in the background of a deficiency of its cognate sensor kinase affects the regulation of genes, including its target ones. The genome-wide effect on gene expression caused by the overproduction was revealed by DNA microarray analysis. In the present work we newly analyzed 24 two-component systems by means of this strategy, leaving out 8 systems to which it was unlikely to be applicable. This analysis revealed various target gene candidates for these two-component systems. It is especially notable that interesting interactions appeared to take place between several two-component systems. Moreover, the probable functions of some unknown two-component systems were deduced from the list of their target gene candidates. This work is heuristic but provides valuable information for further study toward a comprehensive understanding of the B. subtilis two-component regulatory systems. The DNA microarray data obtained in this work are available at the KEGG Expression Database website (http://www.genome.ad.jp/kegg/expression).
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页码:7365 / 7370
页数:6
相关论文
共 27 条
  • [1] Molecular basis of thermosensing:: a two-component signal transduction thermometer in Bacillus subtilis
    Aguilar, PS
    Hernandez-Arriaga, AM
    Cybulski, LE
    Erazo, AC
    de Mendoza, D
    [J]. EMBO JOURNAL, 2001, 20 (07) : 1681 - 1691
  • [2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
    Altschul, SF
    Madden, TL
    Schaffer, AA
    Zhang, JH
    Zhang, Z
    Miller, W
    Lipman, DJ
    [J]. NUCLEIC ACIDS RESEARCH, 1997, 25 (17) : 3389 - 3402
  • [3] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS
    ANAGNOSTOPOULOS, C
    SPIZIZEN, J
    [J]. JOURNAL OF BACTERIOLOGY, 1961, 81 (05) : 741 - &
  • [4] Fabret C, 1998, J BACTERIOL, V180, P6375
  • [5] Two-component signal transduction in Bacillus subtilis:: How one organism sees its world
    Fabret, C
    Feher, VA
    Hoch, JA
    [J]. JOURNAL OF BACTERIOLOGY, 1999, 181 (07) : 1975 - 1983
  • [6] The transcriptional profile of early to middle sporulation in Bacillus subtilis
    Fawcett, P
    Eichenberger, P
    Losick, R
    Youngman, P
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) : 8063 - 8068
  • [7] DUAL CHEMOTAXIS SIGNALING PATHWAYS IN BACILLUS-SUBTILIS - A SIGMA(D)-DEPENDENT GENE ENCODES A NOVEL PROTEIN WITH BOTH CHEW AND CHEY HOMOLOGOUS DOMAINS
    FREDRICK, KL
    HELMANN, JD
    [J]. JOURNAL OF BACTERIOLOGY, 1994, 176 (09) : 2727 - 2735
  • [8] The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis
    Fukuchi, K
    Kasahara, Y
    Asai, K
    Kobayashi, K
    Moriya, S
    Ogasawara, N
    [J]. MICROBIOLOGY-UK, 2000, 146 : 1573 - 1583
  • [9] The competence transcription factor of Bacillus subtilis recognizes short A/T-rich sequences arranged in a unique, flexible pattern along the DNA helix
    Hamoen, LW
    Van Werkhoven, AF
    Bijlsma, JJE
    Dubnau, D
    Venema, G
    [J]. GENES & DEVELOPMENT, 1998, 12 (10) : 1539 - 1550
  • [10] KIRSCH ML, 1993, J BIOL CHEM, V268, P18610