Salt-dependent phase behaviour of the phosphatidylinositol 4,5-diphosphate-water system

被引:9
作者
Hirai, M [1 ]
Takizawa, T [1 ]
Yabuki, S [1 ]
Nakata, Y [1 ]
Hirai, T [1 ]
Hayashi, K [1 ]
机构
[1] SHINSHU UNIV,FAC TEXT SCI & TECHNOL,UEDA,NAGANO 386,JAPAN
来源
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS | 1996年 / 92卷 / 09期
关键词
D O I
10.1039/ft9969201493
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We have studied the effect of mono- and di-valent cations on the structure of the phosphatidylinositol 4,5-diphosphate (PIP2)-biological buffer system at pH 7.2. We found that the PIP2 aggregates form prolate ellipsoidal micelles at pH 7.2 and that the addition of a small amount of Ca2+ ions even below 5 mmol dm(-3) ([Ca2+] : [PIP2] = 5/7) induces a drastic structural phase transition from prolate micelles to disordered lamellae. Mg2+ ions induced a similar phase transition around 10 mmol dm(-3) which, however, occurred less sensitively. In contrast to the case of divalent cations, the presence of Na+ ions did not affect the PIP2 micellar structures up to 0.1 mol dm(-3) ([Na+] : [PIP2] = 100/7), indicating that the above structural change of the PIP2 aggregates is not attributable to the elevation of the electrostatic shielding effect between the polar heads. Modelling analysis suggests that the observed phase transition results from an increase of the critical packing parameter of the PIP2 molecule accompanying the dehydration of the PIP2 polar-head region.
引用
收藏
页码:1493 / 1498
页数:6
相关论文
共 39 条
[1]   INOSITOL TRISPHOSPHATE AND DIACYLGLYCEROL - 2 INTERACTING 2ND MESSENGERS [J].
BERRIDGE, MJ .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :159-193
[2]   INOSITOL TRISPHOSPHATE, A NOVEL 2ND MESSENGER IN CELLULAR SIGNAL TRANSDUCTION [J].
BERRIDGE, MJ ;
IRVINE, RF .
NATURE, 1984, 312 (5992) :315-321
[3]   EXPERIMENTAL ISCHEMIA AND POLYPHOSPHOINOSITIDE METABOLISM [J].
BIRNBERGER, AC ;
ELIASSON, SG .
NEUROLOGY, 1970, 20 (04) :356-+
[4]   PHASE-TRANSITIONS AND FLUIDITY CHARACTERISTICS OF LIPIDS AND CELL-MEMBRANES [J].
CHAPMAN, D .
QUARTERLY REVIEWS OF BIOPHYSICS, 1975, 8 (02) :185-235
[5]  
CHAPMAN D., 1967, CHEM PHYS LIPIDS, V1, P445, DOI 10.1016/0009-3084(67)90023-0
[6]   SCANNING CALORIMETRIC EVIDENCE FOR A 3RD PHASE-TRANSITION IN PHOSPHATIDYLCHOLINE BILAYERS [J].
CHEN, SC ;
STURTEVANT, JM ;
GAFFNEY, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1980, 77 (09) :5060-5063
[7]   MOLECULAR-CONFORMATIONS IN SURFACTANT MICELLES [J].
DILL, KA ;
KOPPEL, DE ;
CANTOR, RS ;
DILL, JD ;
BENDEDOUCH, D ;
CHEN, SH .
NATURE, 1984, 309 (5963) :42-45
[8]  
FOLCH J, 1949, J BIOL CHEM, V177, P497
[9]  
Glatter O., 1982, Small Angle X-Ray Scattering
[10]  
Guinier A., 1939, Ann Phys, V12, P161, DOI DOI 10.1051/ANPHYS/193911120161