Multiplex PCR for simultaneous detection of bacteria of the genus Listeria, Listeria monocytogenes, and major serotypes and epidemic clones of L-monocytogenes

被引:124
作者
Chen, Yi [1 ]
Knabel, Stephen J. [1 ]
机构
[1] Penn State Univ, Dept Food Sci, University Pk, PA 16802 USA
关键词
D O I
10.1128/AEM.00961-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A multiplex PCR assay which combines detection of bacteria of the genus Listeria, Listeria monocytogenes serotypes 1/2a and 4b, and epidemic clones 1, 11, and III of L. monocytogenes was developed. The assay provides a rapid, reliable, and inexpensive method for screening and subgrouping this important food-borne pathogen.
引用
收藏
页码:6299 / 6304
页数:6
相关论文
共 18 条
[1]  
*BROAD I HARV MIT, 2006, LIST MON
[2]   THE HOMOLOGOUS AND HETEROLOGOUS REGIONS WITHIN THE IAP-GENE ALLOW GENUS-SPECIFIC AND SPECIES-SPECIFIC IDENTIFICATION OF LISTERIA SPP BY POLYMERASE CHAIN-REACTION [J].
BUBERT, A ;
KOHLER, S ;
GOEBEL, W .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (08) :2625-2632
[3]   Multi-virulence-locus sequence typing identifies single nucleotide polymorphisms which differentiate epidemic clones and outbreak strains of Listeria monocytogenes [J].
Chen, Yi ;
Zhang, Wei ;
Knabel, Stephen J. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2007, 45 (03) :835-846
[4]   A validated PCR-based method to detect Listeria monocytogenes using raw milk as a food model -: Towards an international standard [J].
D'Agostino, M ;
Wagner, M ;
Vazquez-Boland, JA ;
Kuchta, T ;
Karpiskova, R ;
Hoorfar, J ;
Novella, S ;
Scortti, M ;
Ellison, J ;
Murray, A ;
Fernandes, I ;
Kuhn, M ;
Pazlarova, J ;
Heuvelink, A ;
Cook, N .
JOURNAL OF FOOD PROTECTION, 2004, 67 (08) :1646-1655
[5]   New aspects regarding evolution and virulence of Listeria monocytogenes revealed by comparative genomics and DNA arrays [J].
Doumith, M ;
Cazalet, C ;
Simoes, N ;
Frangeul, L ;
Jacquet, C ;
Kunst, F ;
Martin, P ;
Cossart, P ;
Glaser, P ;
Buchrieser, C .
INFECTION AND IMMUNITY, 2004, 72 (02) :1072-1083
[6]   Differentiation of the major Listeria monocytogenes serovars by multiplex PCR [J].
Doumith, M ;
Buchrieser, C ;
Glaser, P ;
Jacquet, C ;
Martin, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (08) :3819-3822
[7]   Genetic markers unique to Listeria monocytogenes serotype 4b differentiate epidemic clone II (hot dog outbreak strains) from other lineages [J].
Evans, MR ;
Swaminathan, B ;
Graves, LM ;
Altermann, E ;
Klaenhammer, TR ;
Fink, RC ;
Kernodle, S ;
Kathariou, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (04) :2383-2390
[8]   LISTERIA-MONOCYTOGENES, A FOOD-BORNE PATHOGEN [J].
FARBER, JM ;
PETERKIN, PI .
MICROBIOLOGICAL REVIEWS, 1991, 55 (03) :476-511
[9]  
Glaser P, 2001, SCIENCE, V294, P849
[10]   Whole-genome sequence of Listeria welshimeri reveals common steps in genome reduction with Listeria innocua as compared to Listeria monocytogenes [J].
Hain, Torsten ;
Steinweg, Christiane ;
Kuenne, Carsten Tobias ;
Billion, Andre ;
Ghai, Rohit ;
Chatterjee, Som Subhra ;
Domann, Eugen ;
Kaerst, Uwe ;
Goesmann, Alexander ;
Bekel, Thomas ;
Bartels, Daniela ;
Kaiser, Olaf ;
Meyer, Folker ;
Puehler, Alfred ;
Weisshaar, Bernd ;
Wehland, Juergen ;
Liang, Chunguang ;
Dandekar, Thomas ;
Lampidis, Robert ;
Kreft, Juergen ;
Goebel, Werner ;
Chakraborty, Trinad .
JOURNAL OF BACTERIOLOGY, 2006, 188 (21) :7405-7415