Detection of DNA adducts in the human endometrium: a lack of evidence

被引：3

作者：

Carmichael, PL ^{[1
]}

Sardar, S

Neven, F

Van Hoof, I

Ugwumadu, A

Bourne, T

Tomas, E

Hellberg, P

Hewer, AJ

Phillips, DH

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Div Biomed Sci, London, England

[2] Klin St Jan, Brussels, Belgium

[3] St George Hosp, Tooting SW17, England

[4] Oulu Univ Hosp, Oulu 90220, Finland

[5] Sahlgrens Univ Hosp, S-41345 Gothenburg, Sweden

[6] Canc Res Inst, Haddow Labs, Surrey, England

来源：

EUROPEAN JOURNAL OF CANCER | 1998年 / 34卷

关键词：

D O I：

10.1016/S0959-8049(98)00124-5

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A study exploring DNA adducts in human endometria, utilising TLC-P-32-postlabelling, found no evidence for adducts in tamoxifen-treated women, however a subsequent Study utilising HPLC-P-32-postlabelling suggested that very low revels could be detected. We have sought to confirm or dispute these findings by reproducing the HPLC methodology at two centres, analysing endometrial DNA from 20 patients treated with 20 mg/day tamoxifen for between 22 and 65 months. Liver DNA isolated from tamoxifen-treated rats was used as a positive control. We found no convincing evidence for tamoxifen-derived DNA adducts in human endometrium. HPLC elution profiles were indistinguishable from those obtained from untreated women and from women taking the analogue toremifene. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：S66 / S67

页数：2