Inhibitory effect of probiotic Escherichia coli strain Nissle 1917 on adhesion to and invasion of intestinal epithelial cells by adherent-invasive E-coli strains isolated from patients with Crohn's disease

被引:149
作者
Boudeau, J
Glasser, AL
Julien, S
Colombel, JF
Darfeuille-Michaud, A
机构
[1] Univ Auvergne, Fac Pharm, Bacteriol Lab, F-63000 Clermont Ferrand, France
[2] INSERM, EPI 0114, Lab Rech Malad Inflammatoires Intestin, Lille, France
关键词
D O I
10.1046/j.1365-2036.2003.01638.x
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Pathogenic adherent-invasive Escherichia coli have been isolated from ileal lesions of Crohn's disease. Aim: To investigate the non-pathogenic E. coli strain Nissle 1917 (Mutaflor) as possible maintenance therapy in inflammatory bowel disease by testing its ability to prevent adherent-invasive E. coli strains from adhering to and invading human intestinal epithelial cells in vitro. Methods: Bacterial adhesion to and invasion of intestinal epithelial cells (Intestine-407) were assessed by counting the colony-forming units. The inhibitory effect of E. coli Nissle 1917 was determined after co-incubation with adherent-invasive E. coli strains or after pre-incubation of the intestinal epithelial cells with this probiotic strain prior to infection with adherent-invasive E. coli strains. Results: Strain Nissle 1917 exhibited dose- and time-dependent adherence to intestinal epithelial cells and inhibited the adhesion and invasion of various adherent-invasive E. coli strains. In co-infection experiments, the inhibitory effect on adherent-invasive E. coli adhesion reached 78-99.9%. Pre-incubation of intestinal epithelial cells with strain Nissle 1917 reduced adherent-invasive E. coli adhesion by 97.2-99.9%. The inhibitory effect on adherent-invasive E. coli invasion paralleled that on adhesion. Conclusion: As strong and significant inhibitory effects on adherent-invasive E. coli adhesion and invasion were observed in co-infection and pre-infection experiments, E. coli Nissle 1917 could be efficient for preventive or curative probiotic therapy in patients with Crohn's disease.
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页码:45 / 56
页数:12
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