Cloning, expression, and characterization of cis-polyprenyl diphosphate synthase from the thermoacidophilic archaeon Sulfolobus acidocaldarius

被引:27
作者
Hemmi, H [1 ]
Yamashita, S [1 ]
Shimoyama, T [1 ]
Nakayama, T [1 ]
Nishino, T [1 ]
机构
[1] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan
关键词
D O I
10.1128/JB.183.1.401-404.2001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
cis-polyprenyl diphosphate synthases are involved in the biosynthesis of the glycosyl carrier lipid in most organisms. However, only little is known about this enzyme of archaea. In this report, we isolated the gene of cis-polyprenyl diphosphate synthase from a thermoacidophilic archaeon, Sulfolobus acidocaldarius, and characterized the recombinant enzyme.
引用
收藏
页码:401 / 404
页数:4
相关论文
共 16 条
[1]   Use of genomics to identify bacterial undecaprenyl pyrophosphate synthetase:: Cloning, expression, and characterization of the essential uppS gene [J].
Apfel, CM ;
Takács, S ;
Fountoulakis, M ;
Stieger, M ;
Keck, W .
JOURNAL OF BACTERIOLOGY, 1999, 181 (02) :483-492
[2]  
CHEN AJ, 1993, J BIOL CHEM, V268, P11002
[3]   PHOSPHOLIPASE A2 ACTIVITY TOWARDS PHOSPHATIDYLCHOLINE IN MIXED MICELLES - SURFACE DILUTION KINETICS AND EFFECT OF THERMOTROPIC PHASE-TRANSITIONS [J].
DENNIS, EA .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 158 (02) :485-493
[4]  
DENNIS EA, 1973, J LIPID RES, V14, P152
[5]  
FUJII H, 1982, BIOCHIM BIOPHYS ACTA, V712, P716
[6]  
HEMMING FW, 1983, BIOSYNTHESIS ISOPREN, V2, P305
[7]  
KOYAMA T, 1998, BIOCH PRINCIPLES MEC
[8]  
LECHNER J, 1985, J BIOL CHEM, V260, P860
[9]  
MATSUOKA S, 1991, J BIOL CHEM, V266, P3464
[10]   GLYCOSYLATION OF SURFACE GLYCOPROTEIN OF HALOBACTERIUM-SALINARIUM VIA A CYCLIC PATHWAY OF LIPID-LINKED INTERMEDIATES [J].
MESCHER, MF ;
STROMINGER, JL .
FEBS LETTERS, 1978, 89 (01) :37-41