Effect of delayed pulsed-wave ultrasound on local pharmacokinetics and pharmacodynamics of vancomycin-loaded acrylic bone cement in vivo

被引:19
作者
Cai, Xun-Zi
Yan, Shi-Gui
Wu, Hao-Bo
He, Rong-Xin
Dai, Xue-Song
Chen, Hai-Xiang
Yan, Rui-Jian
Zhao, Xin-Hua
机构
[1] Zhejiang Univ, Affiliated Hosp 2, Dept Orthopaed Surg, Hangzhou 310009, Peoples R China
[2] Zhejiang Univ, Sch Med, Bone & Joint Res Ctr, Hangzhou 310009, Peoples R China
[3] Zhejiang Univ, Sch Med, Res Inst Microbiol, Hangzhou 310009, Peoples R China
关键词
D O I
10.1128/AAC.01465-06
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study sought to investigate the effect of delayed pulsed-wave ultrasound with low frequency on drug release from and the antimicrobial efficacy of vancomycin-loaded acrylic bone cement in vivo and the possible mechanism of this effect. After the implantation of cement and the inoculation of Staphylococcas aureus into the bilateral hips of rabbits, ultrasound (average intensity, 300 mW/cm(2); frequency, 46.5 kHz; on/off ratio, 20 min/10 min) was applied to animals in the normal ultrasound group (UG(0-12)) from 0 through 12 h after surgery and to those in the delayed-ultrasound group (UG(12-24)) from 12 through 24 h after surgery. The control group (CG) was not exposed to ultrasound. Based on vancomycin concentrations in left hip cavities at projected time intervals, the amount of time during which the local drug concentration exceeded the MIC (T->MIC) in UG(12-24) was significantly prolonged compared with that in either CG or UG(0-12), and the ratios between the areas under the concentration-time curves over 24 h and the MIC for UG(0-12) and UG(12-24) were both increased compared with that for CG. The greatest reductions in bacterial densities in both right hip aspirates and right femoral tissues at 48 h were achieved with UG(12-24). Local hemorrhage in rabbits of UG(0-12) during the 12-h insonation was more severe than that in rabbits of UG(12-24). Of four variables, the T->MIC and the bioacoustic effect were both identified as parameters predictive of the enhancement of the antimicrobial efficacy of cement by ultrasound. Sustained concentrations above the MIC replaced early high maximum concentrations and long-term subtherapeutic release of the drug, provided that ultrasound was not applied until local hemorrhage was relieved. The enhancement of the antimicrobial efficacy of cement by ultrasound may be attributed to the prolonged T->MIC and the bioacoustic effect caused by ultrasound.
引用
收藏
页码:3199 / 3204
页数:6
相关论文
共 25 条
[1]   Treatment of biofilm infections on implants with low-frequency ultrasound and antibiotics [J].
Carmen, JC ;
Roeder, BL ;
Nelson, JL ;
Ogilvie, RLR ;
Robison, RA ;
Schaalje, GB ;
Pitt, WG .
AMERICAN JOURNAL OF INFECTION CONTROL, 2005, 33 (02) :78-82
[2]   Ultrasonically enhanced vancomycin activity against Staphylococcus epidermidis biofilms in vivo [J].
Carmen, JC ;
Roeder, BL ;
Nelson, JL ;
Beckstead, BL ;
Runyan, CM ;
Schaalje, GB ;
Robison, RA ;
Pitt, WG .
JOURNAL OF BIOMATERIALS APPLICATIONS, 2004, 18 (04) :237-245
[3]  
*CLIN LAB STAND I, 2006, PERFORMANCE STANDARD, P118
[4]   The combination of ultrasound with antibiotics released from bone cement decreases the viability of planktonic and biofilm bacteria:: an in vitro study with clinical strains [J].
Ensing, Geert T. ;
Neut, Danielle ;
Horn, Jim R. van ;
van der Mei, Henny C. ;
Busscher, Henk J. .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2006, 58 (06) :1287-1290
[5]   The influence of ultrasound on the release of gentamicin from antibiotic-loaded acrylic beads and bone cements [J].
Ensing, GT ;
Hendriks, JGE ;
Jongsma, JE ;
van Horn, JR ;
van der Mei, HC ;
Busscher, HJ .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2005, 75B (01) :1-5
[6]   Effect of pulsed ultrasound in combination with gentamicin on bacterial viability in biofilms on bone cements in vivo [J].
Ensing, GT ;
Roeder, BL ;
Nelson, JL ;
van Horn, JR ;
van der Mei, HC ;
Busscher, HJ ;
Pitt, WG .
JOURNAL OF APPLIED MICROBIOLOGY, 2005, 99 (03) :443-448
[7]  
HENDEE WR, 2002, MED IMAGING PHYS, P308
[8]   Increased release of gentamicin from acrylic bone cements under influence of low-frequency ultrasound [J].
Hendriks, JGE ;
Ensing, GT ;
van Horn, JR ;
Lubbers, J ;
van der Mei, HC ;
Busscher, HJ .
JOURNAL OF CONTROLLED RELEASE, 2003, 92 (03) :369-374
[9]   The release of gentamicin from acrylic bone cements in a simulated prosthesis-related interfacial gap [J].
Hendriks, JGE ;
Neut, D ;
van Horn, JR ;
van der Mei, HC ;
Busscher, HJ .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2003, 64B (01) :1-5
[10]   Teicoplanin-containing cement spacers for treatment of experimental Staphylococcus aureus joint prosthesis infection [J].
Ismael, F ;
Bléton, R ;
Saleh-Mghir, A ;
Dautrey, S ;
Massias, L ;
Crémieux, AC .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2003, 47 (10) :3365-3367