Effect of the disulfide bridge and the C-terminal extension on the oligomerization of the amyloid peptide ABri implicated in familial British dementia

被引:40
作者
El-Agnaf, OMA
Sheridan, JM
Sidera, C
Siligardi, G
Hussain, R
Haris, PI
Austen, BM
机构
[1] Univ London St Georges Hosp, Sch Med, Dept Surg, Neurodegenerat Unit, London SW17 0RE, England
[2] Kings Coll London, Dept Pharm, London SE1 8WA, England
[3] De Montfort Univ, Dept Biol Sci, Leicester LE1 9BH, Leics, England
关键词
D O I
10.1021/bi002287i
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Familial British dementia (FBD) is a rare neurodegenerative disorder and shares features with Alzheimer's disease, including amyloid plaque deposits, neurofibrillary tangles, neuronal loss, and progressive dementia. Immunohistochemical and biochemical analysis of plaques and vascular amyloid of FED brains revealed that a 4 kDa peptide named ABri is the main component of the highly insoluble amyloid deposits. In FED patients, the ABri peptide is produced as a result of a point mutation in the usual stop codon of the BRI gene. This mutation produces a BRI precursor protein Il amino acids longer than the wild-type protein. Mutant and wild-type precursor proteins both undergo furin cleavage between residues 243 and 244, producing a peptide of 34 amino acids in the case of ABri and 23 amino acids in the case of the wild-type (WT) peptide. Here we demonstrate that the intramolecular disulfide bond in ABri and the C-terminal extension are required to elongate initially formed dimers to oligomers and fibrils. In contrast, the shorter WT peptide did not aggregate under the same conditions. Conformational analyses indicate that the disulfide bond and the C-terminal extension of ABri are required for the formation of beta -sheet structure. Soluble nonfibrillar ABri oligomers were observed prior to the appearance of mature fibrils. A molecular model of ABri containing three beta -strands, and two beta -hairpins annealed by a disulfide bond, has been constructed, and predicts a hydrophobic surface which is instrumental in promoting oligomerization.
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页码:3449 / 3457
页数:9
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