Overexpression of transcription factor OsLFL1 delays flowering time in Oryza sativa

被引：59

作者：

Peng, Ling-Tao ^{[1
,2
]}

Shi, Zhen-Ying ^{[1
]}

Li, Lin ^{[1
]}

Shen, Ge-Zhi ^{[3
]}

Zhang, Jing-Liu ^{[1
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Plant Physiol & Ecol, Natl Key Lab Plant Mol Genet, Shanghai 200032, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

[3] Shanghai Acad Agr Sci, Plant Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China

来源：

JOURNAL OF PLANT PHYSIOLOGY | 2008年 / 165卷 / 08期

关键词：

late flowering; B3 DNA-binding domain; overexpression; OsLFL1; Oryza sativa;

D O I：

10.1016/j.jplph.2007.07.010

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Flowering time is regulated by genetic programs and environment signals in plants. Genetic analysis of flowering time mutants is instrumental in dissecting the regulatory pathways of flower induction. Genotype W378 is a rice (Oryza sativa) late-flowering mutant selected from our collections of T-DNA insertion line. The T-DNA flanking gene in mutant W378 codes OsLFL1 (O. sativa LEC2 and FUSCA3 Like 1), a putative B3 DNA-binding domain-containing transcription factor. In wild-type rice OsLFL1 is expressed exclusively in spikes and young embryos, while in mutant W378 it is ectopically expressed. Introduction of OsLFL1-RNAi into mutant W378 successfully down-regulated OsLFL1 expression and restored flowering to almost normal time, indicating that overexpression of OsLFL1 confers Late flowering for mutant W378. The flowering-promoting gene Ehd1 and its downstream genes are all down-regulated in W378. Thus, overexpression of OsLFL1 might delay the flowering of W378 by repressing the expression of Ehd1. (C) 2007 Elsevier GmbH. All rights reserved.

引用

页码：876 / 885

页数：10

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