Vestibules Are Part of the Substrate Path in the Multidrug Efflux Transporter AcrB of Escherichia coli

被引：38

作者：

Husain, Fasahath ^{[1
]}

Bikhchandani, Mihir ^{[1
]}

Nikaido, Hiroshi ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA

来源：

JOURNAL OF BACTERIOLOGY | 2011年 / 193卷 / 20期

关键词：

PERIPLASMIC DOMAIN; PUMP; RESISTANCE; MECHANISM; PROTEIN; SITE;

D O I：

10.1128/JB.05759-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The path of substrates in the multidrug efflux pump AcrB of Escherichia coli was examined by using labeling with a lipophilic substrate mimic, Bodipy FL maleimide. Four (out of eight) residues in the vestibule bound the dye, suggesting its role in substrate transport, whereas only one (out of nine) residue in the central cavity tested positive.

引用

页码：5847 / 5849

页数：3

共 17 条

[1] Aminoglycosides are captured from both periplasm and cytoplasm by the AcrD multidrug efflux transporter of Escherichia coli [J].

Aires, JR ;

Nikaido, H .

JOURNAL OF BACTERIOLOGY, 2005, 187 (06) :1923-1929

[2] Mutations in the central cavity and periplasmic domain affect efflux activity of the resistance-nodulation-division pump EmhB from Pseudomonas fluorescens cLP6a [J].

Hearn, EM ;

Gray, MR ;

Foght, JM .

JOURNAL OF BACTERIOLOGY, 2006, 188 (01) :115-123

[3] Substrate path in the AcrB multidrug efflux pump of Escherichia coli [J].

Husain, Fasahath ;

Nikaido, Hiroshi .

MOLECULAR MICROBIOLOGY, 2010, 78 (02) :320-330

[4] Interplay between efflux pumps may provide either additive or multiplicative effects on drug resistance [J].

Lee, A ;

Mao, WM ;

Warren, MS ;

Mistry, A ;

Hoshino, K ;

Okumura, R ;

Ishida, H ;

Lomovskaya, O .

JOURNAL OF BACTERIOLOGY, 2000, 182 (11) :3142-3150

[5] Crystal structure of bacterial multidrug efflux transporter AcrB [J].

Murakami, S ;

Nakashima, R ;

Yamashita, E ;

Yamaguchi, A .

NATURE, 2002, 419 (6907) :587-593

[6] Crystal structures of a multidrug transporter reveal a functionally rotating mechanism [J].

Murakami, Satoshi ;

Nakashima, Ryosuke ;

Yamashita, Eiki ;

Matsumoto, Takashi ;

Yamaguchi, Akihito .

NATURE, 2006, 443 (7108) :173-179

[7] Multidrug efflux pumps of gram-negative bacteria [J].

Nikaido, H .

JOURNAL OF BACTERIOLOGY, 1996, 178 (20) :5853-5859

[8]

Nikaido Hiroshi, 2011, Adv Enzymol Relat Areas Mol Biol, V77, P1

[9] Mechanisms of RND multidrug efflux pumps [J].

Nikaido, Hiroshi ;

Takatsuka, Yumiko .

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2009, 1794 (05) :769-781

[10] CAVER:: a new tool to explore routes from protein clefts, pockets and cavities [J].

Petrek, Martin ;

Otyepka, Michal ;

Banas, Pavel ;

Kosinova, Pavlina ;

Koca, Jaroslav ;

Damborsky, Jiri .

BMC BIOINFORMATICS, 2006, 7 (1)

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