Microcontact printing for precise control of nerve cell growth in culture

被引:121
作者
Wheeler, BC [1 ]
Corey, JM
Brewer, GJ
Branch, DW
机构
[1] Univ Illinois, Beckman Inst, Urbana, IL 61801 USA
[2] Univ Illinois, Biophys Program, Urbana, IL 61801 USA
[3] Univ Illinois, Program Neurosci, Urbana, IL 61801 USA
[4] So Illinois Univ, Sch Med, Dept Med Microbiol & Immunol, Springfield, IL 62794 USA
来源
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME | 1999年 / 121卷 / 01期
关键词
D O I
10.1115/1.2798045
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Microcontact printing, facilitated by silane linker chemistry and high-relief stamps, creates precise patterns of proteins, which in turn control growth of hippocampal neurons in culture. This additive, multi-mask technique permits several different molecules to be patterned on the same substrate. The covalent linker technology permits relatively long-term ( two-week) compliance of neurons to the stamped pattern against a polyethylene glycol background. When polylysine was stamped adjacent to a laminin/polylysine mixture, neural somata and dendrites preferred the polylysine while axons prefer the mixture or the border between the two.
引用
收藏
页码:73 / 78
页数:6
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