Structural elucidation of a capsular polysaccharide from a clinical isolate of Bacteroides vulgatus from a patient with Crohn's disease

被引：6

作者：

Hashimoto, M

Kirikae, F

Dohi, T

Kusumoto, S

Suda, Y

Kirikae, T ^{[1
]}

机构：

[1] Int Med Ctr Japan, Res Inst, Shinjuku Ku, Tokyo 1628655, Japan

[2] Osaka Univ, Grad Sch Sci, Suita, Osaka 565, Japan

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 11期

关键词：

Bacteroides vulgatus; capsular polysaccharide; NMR; structure;

D O I：

10.1046/j.1432-1327.2001.02147.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of a capsular polysaccharide (CPS) from a clinical isolate of Bacteroides vulgatus was elucidated. B. vulgatus IMCJ 1204 was isolated from feces of a patient with Crohn's disease. CPS was prepared by pheno/water extraction of the bacterial cells followed by hydrophobic interaction chromatography and then gel filtration chromatography of the extract. The structure of CPS was determined by chemical analysis and NMR spectroscopy including DQF-COSY, TOCSY, ROESY, HSQC-TOCSY, HMQC and HMBC to be a polysaccharide composed of the following repeating unit: -->3)beta -D-Glcp(1 -->6)[alpha -D-GalpNAc(1 -->2) beta -D-Galp(1 -->4)] beta -D-GlcpNAc(1 -->3)alpha -D-Galp(1 -->4)beta -D-Manp(1 -->.

引用

页码：3139 / 3144

页数：6

共 25 条

[1] COLORIMETRIC ANALYSIS OF SUGARS [J].