Isolation, purification and structural investigation of a water-soluble polysaccharide from Solanum lyratum Thunb

被引：56

作者：

Wu, YL ^{[1
]}

Pan, YJ ^{[1
]}

Sun, CR ^{[1
]}

机构：

[1] Zhejiang Univ, Dept Chem, Hangzhou 310027, Peoples R China

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2005年 / 36卷 / 04期

基金：

中国国家自然科学基金;

关键词：

polysaccharide; Solanum lyratum Thunb (SLT); D-glucan;

D O I：

10.1016/j.ijbiomac.2005.06.007

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Polysaccharides, extracted from the herbs of Chinese natural Solanum lyratum Thunb (SLT), which is a traditional Chinese medicine with hot water and 0.04 M sodium hydroxide successively, were fractionated and purified by ion-exchange and gel-filtration chromatography. According to methylation, periodate oxidation, NMR spectroscopy, partial and graded acid and enzymic hydrolysis analysis, the results indicated the D-glucan to be linear and to contain both (1 -> 3)- and (1 -> 4)-linkages. The anomeric NMR and IR spectra measurements confirmed that the sugar residues were beta-glycosidically linked. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：241 / 245

页数：5

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