L1 antibodies block lymph node fibroblastic reticular matrix remodeling In vivo

被引:20
作者
Di Sciullo, G
Donahue, T
Schachner, M
Bogen, SA [1 ]
机构
[1] Boston Univ, Sch Med, Dept Pathol & Lab Med, Boston, MA 02118 USA
[2] Swiss Fed Inst Technol, Dept Neurobiol, CH-8093 Zurich, Switzerland
[3] Univ Hamburg, Zentrum Mol Neurobiol, D-20246 Hamburg, Germany
关键词
lymph node; L1; cell adhesion molecule; fibroblastic reticular system; architecture;
D O I
10.1084/jem.187.12.1953
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
L1 is an immunoglobulin superfamily adhesion molecule highly expressed on neurons and involved in cell motility, neurite outgrowth, axon fasciculation, myelination, and synaptic plasticity. L1 is also expressed by nonneural cells, but its function outside of the nervous system has not been studied extensively. We find that administration of an L1 monoclonal antibody in vivo disrupts the normal remodeling of lymph node reticular matrix during an immune response. Ultrastructural examination reveals that reticular fibroblasts m mice treated with L1 monoclonal antibodies fail to spread and envelop collagen fibers with their cellular processes. The induced defect in the remodeling of the fibroblastic reticular system results in the loss of normal nodal architecture, collapsed cortical sinusoids, and macrophage accumulation in malformed sinuses. Surprisingly, such profound architectural abnormalities have no detectable effects on the primary immune response to protein antigens.
引用
收藏
页码:1953 / 1963
页数:11
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